OBJECTIVE: To observe the effect of platelet-rich plasma (PRP) on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro. METHODS: Human adipose-derived stem cells were obtained by enzymatic digestion and PRP was prepared by dual centrifugal method. The ADSCS were interfused with 5%, 10%, and 20% PRP in conditioned culture media, using the untreated cells as the control group. The morphology of the cells were observed and their proliferative ability was detected using XTT colorimetric assay. The adipogenic differentiation ability of the cells was evaluated using oil Red O staining. RESULTS: The ADSCS treated with PRP showed better morphology with higher density than the control cells. XTT colorimetric assay demonstrated obviously stronger proliferative activity of PRP-treated cells than the control group (P<0.01). Interfusion with PRP caused a significant increase in adipogenic differentiation of the cells as compared to the control cells (P<0.01). CONCLUSION: PRP treatment produces obvious effects on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.
OBJECTIVE: To observe the effect of platelet-rich plasma (PRP) on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro. METHODS:Human adipose-derived stem cells were obtained by enzymatic digestion and PRP was prepared by dual centrifugal method. The ADSCS were interfused with 5%, 10%, and 20% PRP in conditioned culture media, using the untreated cells as the control group. The morphology of the cells were observed and their proliferative ability was detected using XTT colorimetric assay. The adipogenic differentiation ability of the cells was evaluated using oil Red O staining. RESULTS: The ADSCS treated with PRP showed better morphology with higher density than the control cells. XTT colorimetric assay demonstrated obviously stronger proliferative activity of PRP-treated cells than the control group (P<0.01). Interfusion with PRP caused a significant increase in adipogenic differentiation of the cells as compared to the control cells (P<0.01). CONCLUSION:PRP treatment produces obvious effects on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.