Micromonospora yangpuensis sp. nov., isolated from a sponge.

An actinomycete, strain FXJ6.011(T), was isolated from a cup-shaped sponge collected at Dachan reef, Yangpu in the South China Sea. The strain had morphological characteristics of members of the family Micromonosporaceae. Phylogenetic analysis of the 16S rRNA gene sequence of strain FXJ6.011(T) indicated the highest similarity (98.7 %) to Micromonospora auratinigra JCM 12357(T), Micromonospora chaiyaphumensis JCM 12873(T) and Micromonospora echinofusca JCM 3327(T). Analysis of the gyrB gene sequence also showed that strain FXJ6.011(T) should be assigned to the genus Micromonospora. It contained DD-diaminopimelic acid as the major cell-wall diamino acid and MK-10(H(2)) as predominant menaquinone. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidyl mannosides and phosphatidylinositol dimannoside. The major cellular fatty acids were iso-C(16 : 0), C(17 : 1)ω8c and C(16 : 0). Physiological and biochemical data and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Based on phenotypic and genotypic data, strain FXJ6.011(T) represents a novel Micromonospora species, for which the name Micromonospora yangpuensis sp. nov. is proposed; the type strain is FXJ6.011(T) ( = CGMCC 4.5736(T) = NBRC 107727(T)).