| Literature DB >> 2138458 |
Abstract
A new continuous coupled fluorimetric assay is described for ATPases in general. Thus phosphate released from ATP hydrolysis is coupled to the nucleoside phosphorylase reaction using 7-methylguanosine as a fluorescent substrate for the nucleoside phosphorylase reaction. The hydrolysis of 7-methylguanosine leads to 7-methylguanine, which has lower quantum yield and hence can be used to monitor ATP hydrolysis continuously. The method has the potential to be extended to GTPase and nucleotidyltransferase assays.Entities:
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Year: 1990 PMID： 2138458 PMCID： PMC1131175
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857