Literature DB >> 2138453

Inhibition of cytochalasin D-stimulated G-actin ATPase by ADP-ribosylation with Clostridium perfringens iota toxin.

U Geipel1, I Just, K Aktories.   

Abstract

Clostridium perfringens iota toxin belongs to a novel family of actin-ADP-ribosylating toxins. The effects of ADP-ribosylation of skeletal muscle actin by Clostridium perfringens iota toxin on cytochalasin D-stimulated actin ATPase activity was studied. Cytochalasin D stimulated actin-catalysed ATP hydrolysis maximally by about 30-fold. ADP-ribosylation of actin completely inhibited cytochalasin D-stimulated ATP hydrolysis. Inhibition of ATPase activity occurred at actin concentrations below the critical concentration (0.1 microM), at low concentrations of Mg2+ (50 microM) and even in the actin-DNAase I complex, indicating that ADP-ribosylation of actin blocks the ATPase activity of monomeric actin and that the inhibitory effect is not due to inhibition of the polymerization of actin.

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Year:  1990        PMID： 2138453      PMCID： PMC1131136          DOI： 10.1042/bj2660335

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

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8.  The complex of actin and deoxyribonuclease I as a model system to study the interactions of nucleotides, cations and cytochalasin D with monomeric actin.

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Review 9.  Structural and functional relationships of guanosine triphosphate binding proteins.

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  9 in total

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5.  Cytotoxic effects by microinjection of ADP-ribosylated skeletal muscle G-actin in PtK2 cells in the absence of Clostridium perfringens iota toxin.

Authors:  G Kiefer; M Lerner; P Sehr; I Just; K Aktories
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6.  ADP-ribosylation of Drosophila indirect-flight-muscle actin and arthrin by Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin.

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Review 8.  Clostridial ADP-ribosylating toxins: effects on ATP and GTP-binding proteins.

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Review 9.  Actin-Associated Proteins and Small Molecules Targeting the Actin Cytoskeleton.

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  9 in total

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