Literature DB >> 2137554

Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

V K Parnaik1, P K Kennady.   

Abstract

A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.

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Year:  1990        PMID： 2137554      PMCID： PMC361022          DOI： 10.1128/mcb.10.3.1287-1292.1990

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  35 in total

1.  Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.

Authors:  S Subramani; R Mulligan; P Berg
Journal:  Mol Cell Biol       Date:  1981-09       Impact factor: 4.272

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Characterization of the major polypeptides of the rat liver nuclear envelope.

Authors:  S H Kaufmann; W Gibson; J H Shaper
Journal:  J Biol Chem       Date:  1983-02-25       Impact factor: 5.157

4.  Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.

Authors:  S L McKnight; E R Gavis
Journal:  Nucleic Acids Res       Date:  1980-12-20       Impact factor: 16.971

5.  Characterization of cells transformed by Ad5/Ad12 hybrid early region I plasmids.

Authors:  R Bernards; A Houweling; P I Schrier; J L Bos; A J Van der Eb
Journal:  Virology       Date:  1982-07-30       Impact factor: 3.616

6.  Structure and genomic organization of the mouse dihydrofolate reductase gene.

Authors:  J H Nunberg; R J Kaufman; A C Chang; S N Cohen; R T Schimke
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

7.  Targeting of E. coli beta-galactosidase to the nucleus in yeast.

Authors:  M N Hall; L Hereford; I Herskowitz
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

8.  Molecular cloning of a cDNA specific for the murine p53 cellular tumor antigen.

Authors:  M Oren; A J Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

9.  In vitro translation products specified by the transforming region of adenovirus type 2.

Authors:  D N Halbert; D J Spector; H J Raskas
Journal:  J Virol       Date:  1979-09       Impact factor: 5.103

10.  A large particle associated with the perimeter of the nuclear pore complex.

Authors:  P N Unwin; R A Milligan
Journal:  J Cell Biol       Date:  1982-04       Impact factor: 10.539
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  2 in total

1.  Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells.

Authors:  F D Goodrum; T Shenk; D A Ornelles
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

2.  A cytoplasmically anchored nuclear protein interferes specifically with the import of nuclear proteins but not U1 snRNA.

Authors:  K van Zee; A Dickmanns; U Fischer; R Lührmann; E Fanning
Journal:  J Cell Biol       Date:  1993-04       Impact factor: 10.539
  2 in total

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