OBJECTIVE: At present, circulating markers characterizing the inflammatory infiltration of white adipose tissue (WAT) in human obesity are not well known. We previously identified, by a pangenomic approach (microarrays), the urokinase plasminogen activator receptor (PLAUR or CD87) as a potential marker of subcutaneous adipose tissue macrophage infiltration (ATM). METHOD: We studied i) the presence of PLAUR protein in WAT; ii) the PLAUR amount in plasma of obese patients; iii) the circulating variations during surgery-induced weight loss, and iv) the correlations between PLAUR circulating levels and bioclinical parameters. RESULTS: We observed that PLAUR is preferentially expressed by infiltrating ATMs, with a typical localization on macrophage membrane. Circulating soluble PLAUR levels were significantly elevated in obese patients compared to lean controls. However, despite a trend towards a decrease 3 months after weight loss, PLAUR plasma levels were not modulated during a 1-year weight loss follow-up, suggesting the contribution of secretion sites other than subcutaneous WAT in obese patients. CONCLUSIONS: These findings indicate that PLAUR mRNA expression could be used for the estimation of local subcutaneous ATMs infiltration in obese patients, but it cannot be used as a systemic marker of this inflammatory infiltration in dynamic phases of weight loss.
OBJECTIVE: At present, circulating markers characterizing the inflammatory infiltration of white adipose tissue (WAT) in humanobesity are not well known. We previously identified, by a pangenomic approach (microarrays), the urokinase plasminogen activator receptor (PLAUR or CD87) as a potential marker of subcutaneous adipose tissue macrophage infiltration (ATM). METHOD: We studied i) the presence of PLAUR protein in WAT; ii) the PLAUR amount in plasma of obesepatients; iii) the circulating variations during surgery-induced weight loss, and iv) the correlations between PLAUR circulating levels and bioclinical parameters. RESULTS: We observed that PLAUR is preferentially expressed by infiltrating ATMs, with a typical localization on macrophage membrane. Circulating soluble PLAUR levels were significantly elevated in obesepatients compared to lean controls. However, despite a trend towards a decrease 3 months after weight loss, PLAUR plasma levels were not modulated during a 1-year weight loss follow-up, suggesting the contribution of secretion sites other than subcutaneous WAT in obesepatients. CONCLUSIONS: These findings indicate that PLAUR mRNA expression could be used for the estimation of local subcutaneous ATMs infiltration in obesepatients, but it cannot be used as a systemic marker of this inflammatory infiltration in dynamic phases of weight loss.
Authors: Emma Macdonald; Margaret Cassidy; Bradford E Hall; Sijung Yun; Matthew R Sapio; Pradipta Ray; Megan Doty; Pranavi Nara; Michael D Burton; Stephanie Shiers; Abhik Ray-Chaudhury; Andrew J Mannes; Theodore J Price; Michael J Iadarola; Ashok B Kulkarni Journal: Sci Rep Date: 2022-03-18 Impact factor: 4.996