BACKGROUND: There is increasing evidence of B-cell involvement in the pathogenesis of multiple sclerosis (MS). B-cell activating factor (BAFF) has an essential role in B-cell homeostasis. The chemokine CXCL13 has an important role in the formation and maintenance of B-cell follicles. OBJECTIVE: To measure BAFF and CXCL13 levels in the cerebrospinal fluid (CSF) of patients with MS compared to patients with other neurological diseases. METHODS: Cytokine/chemokine levels were measured by an enzyme-linked immunosorbent assay (ELISA). RESULTS: In MS patients, BAFF levels were highest in patients with secondary progressive disease, and were higher during relapse in patients with relapsing-remitting and secondary progressive disease. CXCL13 levels were also higher during relapse. There was a positive correlation between CXCL13 and the IgG index, and an inverse correlation between BAFF and the IgG index. The implications of this finding are discussed. CONCLUSION: During relapse, we found various positive correlations between BAFF, CXCL13 and the cytokines IL-6 and IL-10. These findings show that molecules that are essential for B-cell recruitment, survival, maturation and function may be working in concert to affect B-cell homeostasis in MS and contribute to the pathophysiology of the disease.
BACKGROUND: There is increasing evidence of B-cell involvement in the pathogenesis of multiple sclerosis (MS). B-cell activating factor (BAFF) has an essential role in B-cell homeostasis. The chemokine CXCL13 has an important role in the formation and maintenance of B-cell follicles. OBJECTIVE: To measure BAFF and CXCL13 levels in the cerebrospinal fluid (CSF) of patients with MS compared to patients with other neurological diseases. METHODS: Cytokine/chemokine levels were measured by an enzyme-linked immunosorbent assay (ELISA). RESULTS: In MSpatients, BAFF levels were highest in patients with secondary progressive disease, and were higher during relapse in patients with relapsing-remitting and secondary progressive disease. CXCL13 levels were also higher during relapse. There was a positive correlation between CXCL13 and the IgG index, and an inverse correlation between BAFF and the IgG index. The implications of this finding are discussed. CONCLUSION: During relapse, we found various positive correlations between BAFF, CXCL13 and the cytokines IL-6 and IL-10. These findings show that molecules that are essential for B-cell recruitment, survival, maturation and function may be working in concert to affect B-cell homeostasis in MS and contribute to the pathophysiology of the disease.
Authors: Erica L Eggers; Brady A Michel; Hao Wu; Sheng-Zhi Wang; Carolyn J Bevan; Aya Abounasr; Natalie S Pierson; Antje Bischof; Max Kazer; Elizabeth Leitner; Ariele L Greenfield; Stanislas Demuth; Michael R Wilson; Roland G Henry; Bruce Ac Cree; Stephen L Hauser; H-Christian von Büdingen Journal: JCI Insight Date: 2017-11-16
Authors: Sharon Chiang; Navin K Kesari; Anthony Bradshaw; Wendy Chen; Rohini Samudralwar; Ammar M Alobaidy; Joseph S Kass Journal: Neurology Date: 2017-10-24 Impact factor: 9.910
Authors: Finn Sellebjerg; Diego Cadavid; Deborah Steiner; Luisa Maria Villar; Richard Reynolds; Daniel Mikol Journal: Ther Adv Neurol Disord Date: 2016-01 Impact factor: 6.570