Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system.

Literature DB >> 21368278

Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system.

Chun-Yuan Ting¹, Stephanie Gu, Sudha Guttikonda, Tzu-Yang Lin, Benjamin H White, Chi-Hon Lee.

Abstract

Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.

Entities: Gene Species

Mesh：

Substances：

Year: 2011 PMID： 21368278 PMCID： PMC3120155 DOI： 10.1534/genetics.110.126193

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

10 in total

Review 1. GAL4 system in Drosophila: a fly geneticist's Swiss army knife.

Authors: Joseph B Duffy
Journal: Genesis Date: 2002 Sep-Oct Impact factor: 2.487

2. Conversion of lacZ enhancer trap lines to GAL4 lines using targeted transposition in Drosophila melanogaster.

Authors: K J Sepp; V J Auld
Journal: Genetics Date: 1999-03 Impact factor: 4.562

3. Refined LexA transactivators and their use in combination with the Drosophila Gal4 system.

Authors: Ryohei Yagi; Franz Mayer; Konrad Basler
Journal: Proc Natl Acad Sci U S A Date: 2010-08-30 Impact factor: 11.205

4. A genetic mosaic approach for neural circuit mapping in Drosophila.

Authors: Rudolf A Bohm; William P Welch; Lindsey K Goodnight; Logan W Cox; Leah G Henry; Tyler C Gunter; Hong Bao; Bing Zhang
Journal: Proc Natl Acad Sci U S A Date: 2010-09-01 Impact factor: 11.205

5. Genetic mosaic with dual binary transcriptional systems in Drosophila.

Authors: Sen-Lin Lai; Tzumin Lee
Journal: Nat Neurosci Date: 2006-04-02 Impact factor: 24.884

6. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression.

Authors: Haojiang Luan; Nathan C Peabody; Charles R Vinson; Benjamin H White
Journal: Neuron Date: 2006-11-09 Impact factor: 17.173

7. Refinement of tools for targeted gene expression in Drosophila.

Authors: Barret D Pfeiffer; Teri-T B Ngo; Karen L Hibbard; Christine Murphy; Arnim Jenett; James W Truman; Gerald M Rubin
Journal: Genetics Date: 2010-08-09 Impact factor: 4.562

8. The neural substrate of spectral preference in Drosophila.

Authors: Shuying Gao; Shin-Ya Takemura; Chun-Yuan Ting; Songling Huang; Zhiyuan Lu; Haojiang Luan; Jens Rister; Andreas S Thum; Meiluen Yang; Sung-Tae Hong; Jing W Wang; Ward F Odenwald; Benjamin H White; Ian A Meinertzhagen; Chi-Hon Lee
Journal: Neuron Date: 2008-10-23 Impact factor: 17.173

9. Conserved role of the Vsx genes supports a monophyletic origin for bilaterian visual systems.

Authors: Ted Erclik; Volker Hartenstein; Howard D Lipshitz; Roderick R McInnes
Journal: Curr Biol Date: 2008-08-28 Impact factor: 10.834

10. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis.

Authors: Christopher J Potter; Bosiljka Tasic; Emilie V Russler; Liang Liang; Liqun Luo
Journal: Cell Date: 2010-04-30 Impact factor: 41.582

10 in total

23 in total

Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system.

Review 1. GAL4 system in Drosophila: a fly geneticist's Swiss army knife.

2. Conversion of lacZ enhancer trap lines to GAL4 lines using targeted transposition in Drosophila melanogaster.

3. Refined LexA transactivators and their use in combination with the Drosophila Gal4 system.

4. A genetic mosaic approach for neural circuit mapping in Drosophila.

5. Genetic mosaic with dual binary transcriptional systems in Drosophila.

6. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression.

7. Refinement of tools for targeted gene expression in Drosophila.

8. The neural substrate of spectral preference in Drosophila.

9. Conserved role of the Vsx genes supports a monophyletic origin for bilaterian visual systems.

10. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis.

1. Cholinergic circuits integrate neighboring visual signals in a Drosophila motion detection pathway.

Review 2. Methods for studying planar cell polarity.

3. Mapping chromatic pathways in the Drosophila visual system.

Review 4. Current techniques for high-resolution mapping of behavioral circuits in Drosophila.

Review 5. Recent Advances in the Genetic Dissection of Neural Circuits in Drosophila.

Review 6. Power tools for gene expression and clonal analysis in Drosophila.

Review 7. Insights from intoxicated Drosophila.

8. Optogenetics in Drosophila.

9. Optogenetic manipulation of neural circuits and behavior in Drosophila larvae.

Review 10. Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster.