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Literature DB >> 21357751

The splice is right: guarantors of fidelity in pre-mRNA splicing.

Abstract

Two recent papers, one from the Staley laboratory (Koodathingal and colleagues) and the other from the Cheng laboratory (Tseng and colleagues), show that the RNA-dependent ATPase Prp16, which is required for the second step of splicing, acts to reject slowly splicing pre-mRNAs immediately before the first catalytic reaction in pre-mRNA splicing. The results answer long-investigated questions about the actions of Prp16 and provide a wealth of molecular details on the proofreading process in pre-mRNA splicing. The discussion here reviews and integrates the results of the two papers and describes the implications for proofreading in splicing.

Entities: Gene

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Year: 2011 PMID： 21357751 PMCID： PMC3062167 DOI： 10.1261/rna.2577511

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

23 in total

1. Spliceosome discards intermediates via the DEAH box ATPase Prp43p.

Authors: Rabiah M Mayas; Hiroshi Maita; Daniel R Semlow; Jonathan P Staley
Journal: Proc Natl Acad Sci U S A Date: 2010-05-12 Impact factor: 11.205

2. DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps.

Authors: Chi-Kang Tseng; Hsueh-Lien Liu; Soo-Chen Cheng
Journal: RNA Date: 2010-11-22 Impact factor: 4.942

3. PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome.

Authors: B Schwer; C Guthrie
Journal: Nature Date: 1991-02-07 Impact factor: 49.962

4. Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome.

Authors: S L Yean; G Wuenschell; J Termini; R J Lin
Journal: Nature Date: 2000-12-14 Impact factor: 49.962

5. The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing.

Authors: Prakash Koodathingal; Thaddeus Novak; Joseph A Piccirilli; Jonathan P Staley
Journal: Mol Cell Date: 2010-08-13 Impact factor: 17.970

6. A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.

Authors: S M Burgess; C Guthrie
Journal: Cell Date: 1993-07-02 Impact factor: 41.582

7. Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants.

Authors: Charles C Query; Maria M Konarska
Journal: Mol Cell Date: 2004-05-07 Impact factor: 17.970

8. A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.

Authors: J R Couto; J Tamm; R Parker; C Guthrie
Journal: Genes Dev Date: 1987-07 Impact factor: 11.361

9. How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing.

Authors: Shelly-Ann James; William Turner; Beate Schwer
Journal: RNA Date: 2002-08 Impact factor: 4.942

10. A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis.

Authors: B Schwer; C Guthrie
Journal: EMBO J Date: 1992-12 Impact factor: 11.598

10 in total

Review 1. Splicing fidelity: DEAD/H-box ATPases as molecular clocks.

Authors: Prakash Koodathingal; Jonathan P Staley
Journal: RNA Biol Date: 2013-06-03 Impact factor: 4.652

Review 2. RNA helicases in splicing.

Authors: Olivier Cordin; Jean D Beggs
Journal: RNA Biol Date: 2012-12-10 Impact factor: 4.652

3. Early splicing functions of fission yeast Prp16 and its unexpected requirement for gene Silencing is governed by intronic features.

Authors: Drisya Vijayakumari; Amit Kumar Sharma; Pushpinder Singh Bawa; Rakesh Kumar; Subhashini Srinivasan; Usha Vijayraghavan
Journal: RNA Biol Date: 2019-03-20 Impact factor: 4.652

The splice is right: guarantors of fidelity in pre-mRNA splicing.

1. Spliceosome discards intermediates via the DEAH box ATPase Prp43p.

2. DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps.

3. PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome.

4. Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome.

5. The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing.

6. A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.

7. Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants.

8. A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.

9. How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing.

10. A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis.

Review 1. Splicing fidelity: DEAD/H-box ATPases as molecular clocks.

Review 2. RNA helicases in splicing.

3. Early splicing functions of fission yeast Prp16 and its unexpected requirement for gene Silencing is governed by intronic features.

4. Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.

5. The Arabidopsis ortholog of the DEAH-box ATPase Prp16 influences auxin-mediated development.

Review 6. New insights into the spliceosome by single molecule fluorescence microscopy.

7. Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.

Review 8. Functional roles of protein splicing factors.

Review 9. Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing.

10. The histone variant H2A.Z promotes splicing of weak introns.