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Literature DB >> 21357437

Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.

Daisuke Yamashiro¹, Yutaka Minouchi, Makoto Ashiuchi.

Abstract

The Bacillus subtilis structural gene pgsE was investigated as a tool for extrachromosomal DNA maintenance (EDM). It ameliorated the stability of high-copy-number vectors, regardless of whether they were derived from rolling-cycle or theta-mode replicons, without any selective pressure. This unique EDM phenomenon may occur via a trans-acting mechanism.

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Year: 2011 PMID： 21357437 PMCID： PMC3126358 DOI： 10.1128/AEM.02649-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

22 in total

1. Plasmid copy-number control and better-than-random segregation genes of pSM19035 share a common regulator.

Authors: A B de la Hoz; S Ayora; I Sitkiewicz; S Fernández; R Pankiewicz; J C Alonso; P Ceglowski
Journal: Proc Natl Acad Sci U S A Date: 2000-01-18 Impact factor: 11.205

2. Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation.

Authors: Anton Meinhart; Juan C Alonso; Norbert Sträter; Wolfram Saenger
Journal: Proc Natl Acad Sci U S A Date: 2003-02-05 Impact factor: 11.205

Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.

1. Plasmid copy-number control and better-than-random segregation genes of pSM19035 share a common regulator.

2. Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation.

3. Moonlighting proteins: old proteins learning new tricks.

4. Bacillus subtilis pgsE (Formerly ywtC) stimulates poly-γ-glutamate production in the presence of zinc.

5. Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3' sequences.

6. Isolation of Bacillus subtilis (chungkookjang), a poly-gamma-glutamate producer with high genetic competence.

7. Cloning and expression of subtilisin amylosacchariticus gene.

8. Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein.

9. Replication and segregational stability of Bacillus plasmid pBAA1.

10. Glutamate racemase is an endogenous DNA gyrase inhibitor.

1. A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis.