Yan-Bin Wang1, Jing Liu, Zhao-Xu Yang. 1. Department of Gastroenterology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Abstract
AIM: To investigate the role of intestinal mucosal blood flow (IMBF) and motility in the damage of intestinal mucosal barrier in rats with traumatic brain injury. METHODS: Sixty-four healthy male Wistar rats were divided randomly into two groups: traumatic brain injury (TBI) group (n=32), rats with traumatic brain injury; and control group (n=32), rats with sham-operation. Each group was divided into four subgroups (n=8) as 6, 12, 24 and 48 h after operation. Intestinal motility was measured by the propulsion ratio of a semi-solid colored marker (carbon-ink). IMBF was measured with the laser-Doppler technique. Endotoxin and D-xylose levels in plasma were measured to evaluate the change of intestinal mucosal barrier function following TBI. RESULTS: The level of endotoxin was significantly higher in TBI group than in the control group at each time point (0.382±0.014 EU/mL vs 0.102±0.007 EU/mL, 0.466±0.018 EU/mL vs 0.114±0.021 EU/mL, 0.478±0.029 EU/mL vs 0.112±0.018 EU/mL and 0.412±0.036 EU/mL vs 0.108±0.011 EU/mL, P<0.05). D-xylose concentrations in plasma in TBI group were significantly higher than in the control group (6.68±2.37 mmol/L vs 3.66±1.07 mmol/L, 8.51±2.69 mmol /L vs 3.15±0.95 mmol/L, 11.68±3.24 mmol/L vs 3.78±1.12 mmol/L and 10.23±2.83 mmol/L vs 3.34±1.23 mmol/ L, P<0.05). The IMBF in TBI group was significantly lower than that in the control group (38.5±2.8 PU vs 45.6±4.6 PU, 25.2±3.1 PU vs 48.2±5.3 PU, 21.5±2.7 PU vs 44.9±2.8 PU, 29. 4±3.8 PU vs 46.7±3.2 PU) (P<0.05). Significant decelerations of intestinal propulsion ratio in TBI groups were found compared with the control group (0.48%±0.06% vs 0.62%±0.03%, 0.37%±0.05% vs 0.64%±0.01%, 0.39%±0.07% vs 0.63%±0.05% and 0.46%±0.03% vs 0.65%±0.02%) (P<0.05). CONCLUSION: The intestinal mucosal permeability is increased obviously in TBI rats. Decrease of intestinal motility and IMBF occur early in TBI, both are important pathogenic factors for stress-related damage of the intestinal mucosal barrier in TBI.
AIM: To investigate the role of intestinal mucosal blood flow (IMBF) and motility in the damage of intestinal mucosal barrier in rats with traumatic brain injury. METHODS: Sixty-four healthy male Wistar rats were divided randomly into two groups: traumatic brain injury (TBI) group (n=32), rats with traumatic brain injury; and control group (n=32), rats with sham-operation. Each group was divided into four subgroups (n=8) as 6, 12, 24 and 48 h after operation. Intestinal motility was measured by the propulsion ratio of a semi-solid colored marker (carbon-ink). IMBF was measured with the laser-Doppler technique. Endotoxin and D-xylose levels in plasma were measured to evaluate the change of intestinal mucosal barrier function following TBI. RESULTS: The level of endotoxin was significantly higher in TBI group than in the control group at each time point (0.382±0.014 EU/mL vs 0.102±0.007 EU/mL, 0.466±0.018 EU/mL vs 0.114±0.021 EU/mL, 0.478±0.029 EU/mL vs 0.112±0.018 EU/mL and 0.412±0.036 EU/mL vs 0.108±0.011 EU/mL, P<0.05). D-xylose concentrations in plasma in TBI group were significantly higher than in the control group (6.68±2.37 mmol/L vs 3.66±1.07 mmol/L, 8.51±2.69 mmol /L vs 3.15±0.95 mmol/L, 11.68±3.24 mmol/L vs 3.78±1.12 mmol/L and 10.23±2.83 mmol/L vs 3.34±1.23 mmol/ L, P<0.05). The IMBF in TBI group was significantly lower than that in the control group (38.5±2.8 PU vs 45.6±4.6 PU, 25.2±3.1 PU vs 48.2±5.3 PU, 21.5±2.7 PU vs 44.9±2.8 PU, 29. 4±3.8 PU vs 46.7±3.2 PU) (P<0.05). Significant decelerations of intestinal propulsion ratio in TBI groups were found compared with the control group (0.48%±0.06% vs 0.62%±0.03%, 0.37%±0.05% vs 0.64%±0.01%, 0.39%±0.07% vs 0.63%±0.05% and 0.46%±0.03% vs 0.65%±0.02%) (P<0.05). CONCLUSION: The intestinal mucosal permeability is increased obviously in TBI rats. Decrease of intestinal motility and IMBF occur early in TBI, both are important pathogenic factors for stress-related damage of the intestinal mucosal barrier in TBI.
Authors: Nicolas Cenac; Alex C Chin; Rafael Garcia-Villar; Christel Salvador-Cartier; Laurent Ferrier; Nathalie Vergnolle; André G Buret; Jean Fioramonti; Lionel Bueno Journal: J Physiol Date: 2004-06-11 Impact factor: 5.182
Authors: Thiruma V Arumugam; Naomi Arnold; Lavinia M Proctor; Michelle Newman; Robert C Reid; Karl A Hansford; David P Fairlie; Ian A Shiels; Stephen M Taylor Journal: Br J Pharmacol Date: 2003-07-29 Impact factor: 8.739
Authors: A B Olsen; R A Hetz; H Xue; K R Aroom; D Bhattarai; E Johnson; S Bedi; C S Cox; K Uray Journal: Neurogastroenterol Motil Date: 2013-04-02 Impact factor: 3.598
Authors: Mohd Fahami Nur Azlina; Yusof Kamisah; Kien Hui Chua; Ibrahim Abdel Aziz Ibrahim; Hj Mohd Saad Qodriyah Journal: PLoS One Date: 2015-10-14 Impact factor: 3.240
Authors: Mohd Fahami Nur Azlina; Hj Mohd Saad Qodriyah; Kien Hui Chua; Yusof Kamisah Journal: World J Gastroenterol Date: 2017-08-28 Impact factor: 5.742
Authors: Mohd Fahami Nur Azlina; Yusof Kamisah; Kien Hui Chua; Hj Mohd Saad Qodriyah Journal: Evid Based Complement Alternat Med Date: 2013-07-22 Impact factor: 2.629
Authors: Johannes Enevoldsen; Simon T Vistisen; Klaus Krogh; Jørgen F Nielsen; Karoline Knudsen; Per Borghammer; Henning Andersen Journal: PeerJ Date: 2018-06-06 Impact factor: 2.984