O A Apine1, J P Jadhav. 1. Department of Biotechnology Shivaji University, Kolhapur, India.
Abstract
AIMS: To optimize the medium components for the production of indole-3-acetic acid (IAA) by isolated bacterium Pantoea agglomerans strain PVM. METHODS AND RESULTS: Present study deals with the production of an essential plant hormone IAA by a bacterial isolate P. agglomerans strain PVM identified by 16S rRNA gene sequence analysis. The medium containing 8g l(-1) of meat extract and 1g l(-1) of l-tryptophan (precursor) at optimum pH 7, 30°C and 48-h incubation gave the maximum production of IAA (2·191 g l(-1) ). Effect of IAA synthesized on in vitro root induction in Nicotiana tobacum (leaf) explants was compared with that of control. IAA was characterized by high-performance thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectroscopy. CONCLUSIONS: Pantoea agglomerans strain PVM was a good candidate for the inexpensive and utmost production of IAA in short period, as it requires simple medium (meat extract and l-tryptophan). SIGNIFICANCE AND IMPACT OF THE STUDY: The present report first time showed the rapid, cost-effective and maximum production of IAA. No reports are available on the optimization of particular medium components for the production of IAA. This study demonstrates a novel approach for in vitro root induction in N. tobacum (leaf) explants.
AIMS: To optimize the medium components for the production of indole-3-acetic acid (IAA) by isolated bacterium Pantoea agglomerans strain PVM. METHODS AND RESULTS: Present study deals with the production of an essential plant hormone IAA by a bacterial isolate P. agglomerans strain PVM identified by 16S rRNA gene sequence analysis. The medium containing 8g l(-1) of meat extract and 1g l(-1) of l-tryptophan (precursor) at optimum pH 7, 30°C and 48-h incubation gave the maximum production of IAA (2·191 g l(-1) ). Effect of IAA synthesized on in vitro root induction in Nicotiana tobacum (leaf) explants was compared with that of control. IAA was characterized by high-performance thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectroscopy. CONCLUSIONS:Pantoea agglomerans strain PVM was a good candidate for the inexpensive and utmost production of IAA in short period, as it requires simple medium (meat extract and l-tryptophan). SIGNIFICANCE AND IMPACT OF THE STUDY: The present report first time showed the rapid, cost-effective and maximum production of IAA. No reports are available on the optimization of particular medium components for the production of IAA. This study demonstrates a novel approach for in vitro root induction in N. tobacum (leaf) explants.