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Literature DB >> 21327058

Oligonucleotide recombination: a hidden treasure.

Bryan Swingle¹, Eric Markel, Samuel Cartinhour.

Abstract

In Swingle et al. we demonstrate that it is possible to use recombineering to direct a variety of changes in wild-type bacterial cells without the addition of phage-encoded proteins. This discovery is potentially applicable to biological engineering in a wide variety of bacterial species. Here we describe key features of oligo recombination as it is currently understood, and propose strategies for expanding the utility of oligo recombination for bioengineering.

Entities: Chemical

Keywords: RecET; allelic exchange; bacteria; gene conversion; homologous recombination; lambda red; oligonucleotide recombination; recombineering

Mesh：

Substances：
Oligonucleotides

Year: 2010 PMID： 21327058 PMCID： PMC3026465 DOI： 10.4161/bbug.1.4.12098

Source DB: PubMed Journal: Bioeng Bugs ISSN： 1949-1018

27 in total

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3. Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli.

Authors: Lynn C Thomason; Nina Costantino; Donald L Court
Journal: mBio Date: 2016-09-13 Impact factor: 7.867

3 in total