INTRODUCTION: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. RESULTS: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. DISCUSSION: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. METHODS: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL-1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.
INTRODUCTION: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. RESULTS: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. DISCUSSION: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. METHODS: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL-1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.
Authors: Jennifer Jones; Edward V Loftus; Remo Panaccione; Li-Sheng Chen; Sandra Peterson; Joseph McConnell; Linnea Baudhuin; Karen Hanson; Brian G Feagan; Scott W Harmsen; Alan R Zinsmeister; Emelie Helou; William J Sandborn Journal: Clin Gastroenterol Hepatol Date: 2008-09-17 Impact factor: 11.382
Authors: William E Bennett; Rosbel González-Rivera; Nurmohammad Shaikh; Vincent Magrini; Michelle Boykin; Barbara B Warner; Aaron Hamvas; Phillip I Tarr Journal: J Immunol Methods Date: 2009-08-03 Impact factor: 2.303
Authors: David E Greenberg; Zhi-Dong Jiang; Robert Steffen; Mangala P Verenker; Herbert L DuPont Journal: J Infect Dis Date: 2002-03-19 Impact factor: 5.226
Authors: T Azim; R C Halder; M S Sarker; S Ahmed; J Hamadani; A Chowdhury; F Qadri; M A Salam; R B Sack; M J Albert Journal: Clin Diagn Lab Immunol Date: 1995-07
Authors: Linda D Bobo; Rana E El Feghaly; Yee-Shiuan Chen; Erik R Dubberke; Zhuolin Han; Alexandra H Baker; Jinmei Li; Carey-Ann D Burnham; David B Haslam Journal: Infect Immun Date: 2012-12-21 Impact factor: 3.441
Authors: Daniel A John; Lisa K Williams; Venkateswarlu Kanamarlapudi; Thomas J Humphrey; Thomas S Wilkinson Journal: Front Microbiol Date: 2017-09-29 Impact factor: 5.640
Authors: Amanda D Buskirk; Esther Ndungo; Avital A Shimanovich; Diana Lam; William C Blackwelder; Usman N Ikumapayi; Bing Ma; Helen Powell; Martin Antonio; James P Nataro; James B Kaper; Marcela F Pasetti Journal: mBio Date: 2022-08-04 Impact factor: 7.786
Authors: Jinsheng Yu; M Isabel Ordiz; Jennifer Stauber; Nurmohammad Shaikh; Indi Trehan; Erica Barnell; Richard D Head; Ken Maleta; Phillip I Tarr; Mark J Manary Journal: Cell Mol Gastroenterol Hepatol Date: 2015-12-11