BACKGROUND: We previously identified by flow cytometry a Lineage-CD44+ (Lin-CD44+) subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma (HNSCC). We now correlate clinical and histologic factors with Lin-CD44+ cell frequency. METHODS: The study included 31 patients with HNSCC, of whom 87% had stage IV disease. The frequency of Lin-CD44+ cells and the success of xenografting patient tumors in mice were correlated with clinical and pathologic data. RESULTS: The mean frequency of Lin-CD44+ cells was 25% (0.4%-81%). It was 36% in patients who had recurrence versus 15% for those without recurrence (p = .04). Successful xenograft implantation occurred in 53%. Seventy-five percent of patients with successful xenografts had recurrence versus 21% of patients with unsuccessful xenografts (p = .003). CONCLUSIONS: Successful xenograft implantation and a high frequency of Lin-CD44+ cells correlate with known poor prognostic factors such as advanced T classification and recurrence. These findings may support the stem cell concept in HNSCC.
BACKGROUND: We previously identified by flow cytometry a Lineage-CD44+ (Lin-CD44+) subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma (HNSCC). We now correlate clinical and histologic factors with Lin-CD44+ cell frequency. METHODS: The study included 31 patients with HNSCC, of whom 87% had stage IV disease. The frequency of Lin-CD44+ cells and the success of xenografting patienttumors in mice were correlated with clinical and pathologic data. RESULTS: The mean frequency of Lin-CD44+ cells was 25% (0.4%-81%). It was 36% in patients who had recurrence versus 15% for those without recurrence (p = .04). Successful xenograft implantation occurred in 53%. Seventy-five percent of patients with successful xenografts had recurrence versus 21% of patients with unsuccessful xenografts (p = .003). CONCLUSIONS: Successful xenograft implantation and a high frequency of Lin-CD44+ cells correlate with known poor prognostic factors such as advanced T classification and recurrence. These findings may support the stem cell concept in HNSCC.
Authors: Hui Sun Leong; Fui Teen Chong; Pui Hoon Sew; Dawn P Lau; Bernice H Wong; Bin-Tean Teh; Daniel S W Tan; N Gopalakrishna Iyer Journal: Stem Cells Transl Med Date: 2014-07-14 Impact factor: 6.940
Authors: Osama A Elkashty; Ghada Abu Elghanam; Xinyun Su; Younan Liu; Peter J Chauvin; Simon D Tran Journal: Carcinogenesis Date: 2020-06-17 Impact factor: 4.944
Authors: Alice L Tang; John H Owen; Samantha J Hauff; Jung Je Park; Silvana Papagerakis; Carol R Bradford; Thomas E Carey; Mark E Prince Journal: Otolaryngol Head Neck Surg Date: 2013-04-12 Impact factor: 3.497