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Literature DB >> 21317266

Proof of concept for recombinant cellular controls in quantitative molecular pathogen detection.

Peter Rossmanith¹, Patrick Mester, Karin Frühwirth, Sabine Fuchs, Martin Wagner.

Abstract

In this study, we present the concept of internal sample process controls (ISPCs) to monitor the efficiency of an analytical chain using sample preparation and quantitative PCR (qPCR). A recombinant Listeria monocytogenes ΔprfA (targeted deletion) strain containing a competitive artificial single-copy genomic target was applied to naturally contaminated samples to demonstrate its analytical suitability as an ISPC.

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Mesh：

Year: 2011 PMID： 21317266 PMCID： PMC3067449 DOI： 10.1128/AEM.02601-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

22 in total

1. Diagnostic PCR: making internal amplification control mandatory.

Authors: J Hoorfar; N Cook; B Malorny; M Wagner; D De Medici; A Abdulmawjood; P Fach
Journal: J Appl Microbiol Date: 2004 Impact factor: 3.772

Review 2. Practical considerations in design of internal amplification controls for diagnostic PCR assays.

Authors: J Hoorfar; B Malorny; A Abdulmawjood; N Cook; M Wagner; P Fach
Journal: J Clin Microbiol Date: 2004-05 Impact factor: 5.948

Review 3. Bacterial separation and concentration from complex sample matrices: a review.

Authors: Kelly A Stevens; Lee-Ann Jaykus
Journal: Crit Rev Microbiol Date: 2004 Impact factor: 7.624

Review 4. The challenge to quantify Listeria monocytogenes--a model leading to new aspects in molecular biological food pathogen detection.

Authors: P Rossmanith; M Wagner
Journal: J Appl Microbiol Date: 2010-12-23 Impact factor: 3.772

5. Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters.

Authors: S Di Pasquale; M Paniconi; B Auricchio; L Orefice; A C Schultz; D De Medici
Journal: J Virol Methods Date: 2010-01-25 Impact factor: 2.014

6. Listeriosis outbreak caused by acid curd cheese Quargel , Austria and Germany 2009.

Authors: R Fretz; U Sagel; W Ruppitsch; At Pietzka; A Stoger; S Huhulescu; S Heuberger; J Pichler; P Much; G Pfaff; K Stark; R Prager; A Flieger; O Feenstra; F Allerberger
Journal: Euro Surveill Date: 2010-02-04

7. Differential interaction of the transcription factor PrfA and the PrfA-activating factor (Paf) of Listeria monocytogenes with target sequences.

Authors: C Dickneite; R Böckmann; A Spory; W Goebel; Z Sokolovic
Journal: Mol Microbiol Date: 1998-03 Impact factor: 3.501

8. Specific binding of the Listeria monocytogenes transcriptional regulator PrfA to target sequences requires additional factor(s) and is influenced by iron.

Authors: R Böckmann; C Dickneite; B Middendorf; W Goebel; Z Sokolovic
Journal: Mol Microbiol Date: 1996-11 Impact factor: 3.501

9. A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model--towards an international standard.

Authors: M D'Agostino; M Wagner; J A Vazquez-Boland; T Kuchta; R Karpiskova; J Hoorfar; S Novella; M Scortti; J Ellison; A Murray; I Fernandes; M Kuhn; J Pazlarova; A Heuvelink; N Cook
Journal: J Food Prot Date: 2004-08 Impact factor: 2.077

4. Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue.

Authors: Evelyne Mann; Katharina Pommer; Patrick Mester; Martin Wagner; Peter Rossmanith
Journal: BMC Vet Res Date: 2014-03-03 Impact factor: 2.741

4 in total