OBJECTIVE: Alteration in the vaginal flora has been associated with adverse pregnancy outcomes. The objective of this study was to examine the impact of changes in individual microflora on genital immunity among low-risk pregnant women in early pregnancy. DESIGN: Cross-sectional study. SETTING: Large, tertiary care, academic hospital clinic. POPULATION: Low-risk women were enrolled prior to 14 weeks' gestation. METHODS: Women were included if they had no medical or previous obstetrical complications, were non-smokers, had no sexually transmitted infections and no intercourse in the last 48 hours. Consenting women underwent speculum examination for collection of vaginal culture and Dacron swabs for cytokine analysis. Semi-quantitative vaginal cultures were performed in a reference laboratory. MAIN OUTCOME MEASURES: Concentrations of immune mediators were compared in the presence of various organisms. Concentrations were converted to multiples of the median to standardize the values of each mediator. Regression analyses were performed to control for race. RESULTS: We enrolled 47 women. The frequencies of genital microorganisms were: H(2)O(2) -producing lactobacilli (70%), Ureaplasma urealyticum (66%), Gardnerella vaginalis (45%), anaerobic non-pigmented Gram-negative rods (ANPGNR, 40%), anaerobic pigmented Gram-negative rods (APGNR, 17%). After adjusting for race and body mass index, interleukin-1β, interferon-γ, tumor necrosis factor-α and granulocyte macrophage-colony stimulating factor were increased in the presence of G. vaginalis, ANPGNR, and APGNR. There was no consistent impact on the other markers of immune activation. CONCLUSION: The presence of individual species impacts genital immunity among low-risk pregnant women. Perturbations in genital immunity could partially explain heterogeneity in adverse pregnancy outcomes.
OBJECTIVE: Alteration in the vaginal flora has been associated with adverse pregnancy outcomes. The objective of this study was to examine the impact of changes in individual microflora on genital immunity among low-risk pregnant women in early pregnancy. DESIGN: Cross-sectional study. SETTING: Large, tertiary care, academic hospital clinic. POPULATION: Low-risk women were enrolled prior to 14 weeks' gestation. METHODS:Women were included if they had no medical or previous obstetrical complications, were non-smokers, had no sexually transmitted infections and no intercourse in the last 48 hours. Consenting women underwent speculum examination for collection of vaginal culture and Dacron swabs for cytokine analysis. Semi-quantitative vaginal cultures were performed in a reference laboratory. MAIN OUTCOME MEASURES: Concentrations of immune mediators were compared in the presence of various organisms. Concentrations were converted to multiples of the median to standardize the values of each mediator. Regression analyses were performed to control for race. RESULTS: We enrolled 47 women. The frequencies of genital microorganisms were: H(2)O(2) -producing lactobacilli (70%), Ureaplasma urealyticum (66%), Gardnerella vaginalis (45%), anaerobic non-pigmented Gram-negative rods (ANPGNR, 40%), anaerobic pigmented Gram-negative rods (APGNR, 17%). After adjusting for race and body mass index, interleukin-1β, interferon-γ, tumor necrosis factor-α and granulocyte macrophage-colony stimulating factor were increased in the presence of G. vaginalis, ANPGNR, and APGNR. There was no consistent impact on the other markers of immune activation. CONCLUSION: The presence of individual species impacts genital immunity among low-risk pregnant women. Perturbations in genital immunity could partially explain heterogeneity in adverse pregnancy outcomes.
Authors: Hyagriv N Simhan; Steve N Caritis; Marijane A Krohn; Begoña Martinez de Tejada; Daniel V Landers; Sharon L Hillier Journal: Am J Obstet Gynecol Date: 2003-08 Impact factor: 8.661
Authors: M R Joesoef; S L Hillier; G Wiknjosastro; H Sumampouw; M Linnan; W Norojono; A Idajadi; B Utomo Journal: Am J Obstet Gynecol Date: 1995-11 Impact factor: 8.661
Authors: Harald Leitich; Mathias Brunbauer; Barbara Bodner-Adler; Alexandra Kaider; Christian Egarter; Peter Husslein Journal: Am J Obstet Gynecol Date: 2003-03 Impact factor: 8.661
Authors: S L Hillier; R P Nugent; D A Eschenbach; M A Krohn; R S Gibbs; D H Martin; M F Cotch; R Edelman; J G Pastorek; A V Rao Journal: N Engl J Med Date: 1995-12-28 Impact factor: 91.245
Authors: Caroline Mitchell; Jennifer E Balkus; David Fredricks; Congzhou Liu; Jennifer McKernan-Mullin; Lisa M Frenkel; Christina Mwachari; Amneris Luque; Susan E Cohn; Craig R Cohen; Robert Coombs; Jane Hitti Journal: AIDS Res Hum Retroviruses Date: 2012-11-05 Impact factor: 2.205
Authors: Brenna L Anderson; Hector Mendez-Figueroa; Joshua D Dahlke; Christina Raker; Sharon L Hillier; Susan Cu-Uvin Journal: Am J Obstet Gynecol Date: 2013-01-10 Impact factor: 8.661
Authors: Anita Mitra; David A MacIntyre; Julian R Marchesi; Yun S Lee; Phillip R Bennett; Maria Kyrgiou Journal: Microbiome Date: 2016-11-01 Impact factor: 14.650
Authors: Vijay R Ramakrishnan; Justin Holt; Leah F Nelson; Diana Ir; Charles E Robertson; Daniel N Frank Journal: Allergy Rhinol (Providence) Date: 2018-08-02
Authors: Anita Mitra; David A MacIntyre; George Ntritsos; Ann Smith; Konstantinos K Tsilidis; Julian R Marchesi; Phillip R Bennett; Anna-Barbara Moscicki; Maria Kyrgiou Journal: Nat Commun Date: 2020-04-24 Impact factor: 14.919