Literature DB >> 213049

A general method for purification of deoxycytidine kinase.

A Baxter, L M Currie, J P Durham.   

Abstract

Deoxycytidine kinase from a variety of animal tissues binds to Cibacron Blue 3G-A coupled to Sepharose CL-6B. The enzyme can be selectively eluted with ATP to yield single-step purifications of 17-89-fold. The affinity adsorbent is re-usable.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 213049      PMCID： PMC1185873          DOI： 10.1042/bj1731005

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.

Authors:  S T Thompson; K H Cass; E Stellwagen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

2.  Characterization of the kinase(s) involved in the phosphorylation of - and -2'-deoxythioguanosine.

Authors:  Y Nakai; G A LePage
Journal:  Cancer Res       Date:  1972-11       Impact factor: 12.701

3.  Deoxycytidine kinase. I. Distribution in normal and neoplastic tissues and interrelationships of deoxycytidine and 1-beta-D-arabinofuranosylcytosine phosphorylation.

Authors:  J P Durham; D H Ives
Journal:  Mol Pharmacol       Date:  1969-07       Impact factor: 4.436

4.  Affinity chromatography of phosphofructokinase using Cibacron blue F3G-A.

Authors:  H J Böhme; G Kopperschläger; J Schulz; E Hofmann
Journal:  J Chromatogr       Date:  1972-06-28

5.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

6.  Distinctive molecular forms of thymidine kinase in mitochondria of normal and bromodeoxyuridine-resistant HeLa cells.

Authors:  S Kit; W C Leung; L A Kaplan
Journal:  Eur J Biochem       Date:  1973-11-01

7.  A simple colorimetric method for determination of protein.

Authors:  S Bramhall; N Noack; M Wu; J R Loewenberg
Journal:  Anal Biochem       Date:  1969-10-01       Impact factor: 3.365

8.  Deoxycytidine kinase. II. Purification and general properties of the calf thymus enzyme.

Authors:  J P Durham; D H Ives
Journal:  J Biol Chem       Date:  1970-05-10       Impact factor: 5.157

9.  Cytosine arabinoside in acute granulocytic leukemia.

Authors:  B Goodell; B Leventhal; E Henderson
Journal:  Clin Pharmacol Ther       Date:  1971 Jul-Aug       Impact factor: 6.875

10.  Determination of Cibacron Blue F3gA substitution in blue Sephadex and blue dextran-Sepharose.

Authors:  G K Chambers
Journal:  Anal Biochem       Date:  1977-12       Impact factor: 3.365
View more
  1 in total

1.  Tissue-specific and subcellular localization of phototropin determined by immuno-blotting.

Authors:  Elke Knieb; Michael Salomon; Wolfhart Rüdiger
Journal:  Planta       Date:  2003-11-21       Impact factor: 4.116
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.