A rapid determination of drug candidate tyrosol galactoside in rat plasma by HPLC and its application to the pharmacokinetics study.

A simple and sensitive HPLC method has been developed and validated for the determination of tyrosol galactoside (TG) in rat plasma. After one-step protein precipitation with methanol, plasma samples were separated on an Ultimate AQ-C18 column (150 mm×4.6 mm, 5 μm) using acetonitrile-water (7:93, v/v) as mobile phase at a flow rate of 1.2 mL/min. The ultraviolet detection wavelength was set at 275 nm. The lower limit of quantification was 1.140 μg/mL. The calibration curve was linear over a concentration range of 1.140-228.0 μg/mL. The assay accuracy and precision were within the range of 99.6-103.0 and 2.17-6.23%, respectively. The developed method was successfully applied to the pharmacokinetics study of TG in rats after intravenous and oral administration. The bioavailability of TG in rats is 27.9%.