Literature DB >> 21295271

Critical appraisal of the side population assay in stem cell and cancer stem cell research.

Anna Golebiewska1, Nicolaas H C Brons, Rolf Bjerkvig, Simone P Niclou.   

Abstract

The "Side Population" (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis, and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy, and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21295271     DOI： 10.1016/j.stem.2011.01.007

Source DB:  PubMed          Journal:  Cell Stem Cell        ISSN： 1875-9777            Impact factor:   24.633


  142 in total

1.  Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels.

Authors:  Hisamichi Naito; Hiroyasu Kidoya; Susumu Sakimoto; Taku Wakabayashi; Nobuyuki Takakura
Journal:  EMBO J       Date:  2011-12-16       Impact factor: 11.598

2.  Chondrogenic progenitor cells respond to cartilage injury.

Authors:  Dongrim Seol; Daniel J McCabe; Hyeonghun Choe; Hongjun Zheng; Yin Yu; Keewoong Jang; Morgan W Walter; Abigail D Lehman; Lei Ding; Joseph A Buckwalter; James A Martin
Journal:  Arthritis Rheum       Date:  2012-11

Review 3.  Stem Cells in the Cornea.

Authors:  Andrew J Hertsenberg; James L Funderburgh
Journal:  Prog Mol Biol Transl Sci       Date:  2015-05-27       Impact factor: 3.622

4.  SP/drug efflux functionality of hematopoietic progenitors is controlled by mesenchymal niche through VLA-4/CD44 axis.

Authors:  J-V Malfuson; L Boutin; D Clay; C Thépenier; C Desterke; F Torossian; B Guerton; A Anginot; T de Revel; J-J Lataillade; M-C Le Bousse-Kerdilès
Journal:  Leukemia       Date:  2013-09-03       Impact factor: 11.528

5.  Characterisation of human limbal side population cells isolated using an optimised protocol from an immortalised epithelial cell line and primary limbal cultures.

Authors:  Bakiah Shaharuddin; Ian Harvey; Sajjad Ahmad; Simi Ali; Annette Meeson
Journal:  Stem Cell Rev Rep       Date:  2014-04       Impact factor: 5.739

Review 6.  A high throughput flow cytometric assay platform targeting transporter inhibition.

Authors:  George P Tegos; Annette M Evangelisti; J Jacob Strouse; Oleg Ursu; Cristian Bologa; Larry A Sklar
Journal:  Drug Discov Today Technol       Date:  2014-06

7.  Chronic chemotherapeutic stress promotes evolution of stemness and WNT/beta-catenin signaling in colorectal cancer cells: implications for clinical use of WNT-signaling inhibitors.

Authors:  Meriam Ayadi; Anaïs Bouygues; Djamila Ouaret; Nathalie Ferrand; Salem Chouaib; Jean-Paul Thiery; Christian Muchardt; Michèle Sabbah; Annette K Larsen
Journal:  Oncotarget       Date:  2015-07-30

Review 8.  Cervical cancer stem cells: opportunities and challenges.

Authors:  Ravindresh Chhabra
Journal:  J Cancer Res Clin Oncol       Date:  2015-01-07       Impact factor: 4.553

9.  Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples.

Authors:  Maximilian Boesch; Elisabeth Hoflehner; Dominik Wolf; Guenther Gastl; Sieghart Sopper
Journal:  J Vis Exp       Date:  2017-05-10       Impact factor: 1.355

10.  Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish.

Authors:  Margaret M Pruitt; Wilfredo Marin; Michael R Waarts; Jill L O de Jong
Journal:  J Vis Exp       Date:  2017-05-04       Impact factor: 1.355
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