Literature DB >> 2129226

Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity.

J Nichols1, E P Evans, A G Smith.   

Abstract

The regulatory factor Differentiation Inhibiting Activity/Leukaemia Inhibitory Factor (DIA/LIF) suppresses the differentiation of cultured embryonic stem (ES) cells. In the present study, it is shown that ES cell lines can be derived and maintained in the absence of feeder layers using medium supplemented with purified DIA/LIF. These cells can differentiate normally in vitro and in vivo and they retain the capacity for germ-line transmission. DIA/LIF therefore fulfils the essential function of feeders in the isolation of pluripotential stem cells.

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Year:  1990        PMID： 2129226     DOI： 10.1242/dev.110.4.1341

Source DB:  PubMed          Journal:  Development        ISSN： 0950-1991            Impact factor:   6.868


  77 in total

1.  Retroviral expression in embryonic stem cells and hematopoietic stem cells.

Authors:  S R Cherry; D Biniszkiewicz; L van Parijs; D Baltimore; R Jaenisch
Journal:  Mol Cell Biol       Date:  2000-10       Impact factor: 4.272

2.  A developmental switch in H4 acetylation upstream of Xist plays a role in X chromosome inactivation.

Authors:  L P O'Neill; A M Keohane; J S Lavender; V McCabe; E Heard; P Avner; N Brockdorff; B M Turner
Journal:  EMBO J       Date:  1999-05-17       Impact factor: 11.598

Review 3.  Extrinsic regulation of pluripotent stem cells.

Authors:  Martin F Pera; Patrick P L Tam
Journal:  Nature       Date:  2010-06-10       Impact factor: 49.962

4.  An ES-like cell line from the marine fish Sparus aurata: characterization and chimaera production.

Authors:  Julia Béjar; Yunhan Hong; M Carmen Alvarez
Journal:  Transgenic Res       Date:  2002-06       Impact factor: 2.788

5.  Optimization of a serum-free culture medium for mouse embryonic stem cells using design of experiments (DoE) methodology.

Authors:  Fanny Knöspel; Rudolf K Schindler; Marc Lübberstedt; Stephanie Petzolt; Jörg C Gerlach; Katrin Zeilinger
Journal:  Cytotechnology       Date:  2010-09-22       Impact factor: 2.058

6.  Evidence for calreticulin attenuation of cardiac hypertrophy induced by pressure overload and soluble agonists.

Authors:  Sylvia Papp; Ewa Dziak; Golam Kabir; Peter Backx; Sophie Clement; Michal Opas
Journal:  Am J Pathol       Date:  2010-01-28       Impact factor: 4.307

7.  Epigenetic regulation of Atoh1 guides hair cell development in the mammalian cochlea.

Authors:  Zlatka P Stojanova; Tao Kwan; Neil Segil
Journal:  Development       Date:  2015-10-15       Impact factor: 6.868

8.  Smek promotes histone deacetylation to suppress transcription of Wnt target gene brachyury in pluripotent embryonic stem cells.

Authors:  Jungmook Lyu; Eek-Hoon Jho; Wange Lu
Journal:  Cell Res       Date:  2011-03-22       Impact factor: 25.617

9.  Basal medium composition and serum or serum replacement concentration influences on the maintenance of murine embryonic stem cells.

Authors:  Muhammad A Chaudhry; Timothy Z Vitalis; Bruce D Bowen; James M Piret
Journal:  Cytotechnology       Date:  2008-12-20       Impact factor: 2.058

10.  Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates.

Authors:  Miao-Hsueh Chen; Ya-Jun Li; Takatoshi Kawakami; Shan-Mei Xu; Pao-Tien Chuang
Journal:  Genes Dev       Date:  2004-03-15       Impact factor: 11.361
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