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Literature DB >> 21281565

Oligomerization state of dynamin 2 in cell membranes using TIRF and number and brightness analysis.

Justin A Ross¹, Michelle A Digman², Lei Wang³, Enrico Gratton², Joseph P Albanesi³, David M Jameson⁴.

Abstract

Dynamin 2 is an ubiquitously expressed ∼100 kDa GTPase involved in receptor-mediated endocytosis, Golgi budding, and cytoskeletal reorganization. Dynamin molecules assemble around the necks of budding vesicles and constrict membranes in a GTP-dependent process, resulting in vesicle release. The oligomerization state of dynamin 2 in the membrane is still controversial. We investigated dynamin 2 within the plasma membrane of live cells using total internal reflection microscopy coupled with number and brightness analysis. Our results demonstrate that dynamin 2 is primarily tetrameric throughout the entire cell membrane, aside from punctate structures that may correspond to regions of membrane vesiculation.

Entities: Chemical Gene

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Year: 2011 PMID： 21281565 PMCID： PMC3030261 DOI： 10.1016/j.bpj.2010.12.3703

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

12 in total

Review 1. The dynamin superfamily: universal membrane tubulation and fission molecules?

Authors: Gerrit J K Praefcke; Harvey T McMahon
Journal: Nat Rev Mol Cell Biol Date: 2004-02 Impact factor: 94.444

2. Phosphatidylinositol (4,5)-bisphosphate-dependent activation of dynamins I and II lacking the proline/arginine-rich domains.

Authors: H C Lin; B Barylko; M Achiriloaie; J P Albanesi
Journal: J Biol Chem Date: 1997-10-10 Impact factor: 5.157

3. The dynamin middle domain is critical for tetramerization and higher-order self-assembly.

Authors: Rajesh Ramachandran; Mark Surka; Joshua S Chappie; Douglas M Fowler; Ted R Foss; Byeong Doo Song; Sandra L Schmid
Journal: EMBO J Date: 2006-12-14 Impact factor: 11.598

4. Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode.

Authors: Rooshin B Dalal; Michelle A Digman; Alan F Horwitz; Valeria Vetri; Enrico Gratton
Journal: Microsc Res Tech Date: 2008-01 Impact factor: 2.769

5. Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane.

Authors: Rajesh Ramachandran; Sandra L Schmid
Journal: EMBO J Date: 2007-12-13 Impact factor: 11.598

6. Membrane curvature controls dynamin polymerization.

Authors: Aurélien Roux; Gerbrand Koster; Martin Lenz; Benoît Sorre; Jean-Baptiste Manneville; Pierre Nassoy; Patricia Bassereau
Journal: Proc Natl Acad Sci U S A Date: 2010-02-16 Impact factor: 11.205

7. The mechanism of GTP hydrolysis by dynamin II: a transient kinetic study.

Authors: D D Binns; M K Helms; B Barylko; C T Davis; D M Jameson; J P Albanesi; J F Eccleston
Journal: Biochemistry Date: 2000-06-20 Impact factor: 3.162

8. Domain structure and intramolecular regulation of dynamin GTPase.

Authors: A B Muhlberg; D E Warnock; S L Schmid
Journal: EMBO J Date: 1997-11-17 Impact factor: 11.598

9. Three-dimensional reconstruction of dynamin in the constricted state.

Authors: P Zhang; J E Hinshaw
Journal: Nat Cell Biol Date: 2001-10 Impact factor: 28.824

10. Mapping the number of molecules and brightness in the laser scanning microscope.

Authors: Michelle A Digman; Rooshin Dalal; Alan F Horwitz; Enrico Gratton
Journal: Biophys J Date: 2007-12-20 Impact factor: 4.033

23 in total

1. Oligomer size of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor revealed by fluorescence correlation spectroscopy with photon counting histogram analysis: evidence for homodimers without monomers or tetramers.

Authors: Katharine Herrick-Davis; Ellinor Grinde; Tara Lindsley; Ann Cowan; Joseph E Mazurkiewicz
Journal: J Biol Chem Date: 2012-05-16 Impact factor: 5.157

2. A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells.

Authors: Nicholas G James; Michelle A Digman; Justin A Ross; Barbara Barylko; Lei Wang; Jinhui Li; Yan Chen; Joachim D Mueller; Enrico Gratton; Joseph P Albanesi; David M Jameson
Journal: Biochim Biophys Acta Date: 2013-09-07

3. Dimeric endophilin A2 stimulates assembly and GTPase activity of dynamin 2.

Authors: Justin A Ross; Yan Chen; Joachim Müller; Barbara Barylko; Lei Wang; Hunter B Banks; Joseph P Albanesi; David M Jameson
Journal: Biophys J Date: 2011-02-02 Impact factor: 4.033

Review 4. Characterizing proteins in their cellular environment: Examples of recent advances in quantitative fluorescence microscopy.

Authors: Catherine A Royer
Journal: Protein Sci Date: 2019-05-22 Impact factor: 6.725

Review 5. Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.

Authors: Christina Papini; Catherine A Royer
Journal: Biophys Rev Date: 2018-01-30

6. Quantifying Protein-mRNA Interactions in Single Live Cells.

Authors: Bin Wu; Adina R Buxbaum; Zachary B Katz; Young J Yoon; Robert H Singer
Journal: Cell Date: 2015-07-02 Impact factor: 41.582

7. The Ebola virus matrix protein penetrates into the plasma membrane: a key step in viral protein 40 (VP40) oligomerization and viral egress.

Authors: Emmanuel Adu-Gyamfi; Smita P Soni; Yi Xue; Michelle A Digman; Enrico Gratton; Robert V Stahelin
Journal: J Biol Chem Date: 2013-01-06 Impact factor: 5.157