Literature DB >> 2128089

Simple and rapid method for purification of vinculin from bovine aorta.

R Kobayashi1, Y Tashima.   

Abstract

A new rapid purification procedure has been developed for mammalian smooth muscle vinculin. Bovine aorta vinculin has been purified by a two-step procedure employing hydrophobic chromatography on phenyl-Sepharose and gel filtration. This procedure should be particularly useful for purifying vinculin from tissues in which it is present in a relatively low concentration. In addition, the new purification protocol provides highly pure vinculin free from active contaminants which reduce the low-shear viscosity of F-actin solution.

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Year:  1990        PMID： 2128089     DOI： 10.1007/bf01745214

Source DB:  PubMed          Journal:  J Muscle Res Cell Motil        ISSN： 0142-4319            Impact factor:   2.698


  29 in total

1.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

2.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.

Authors:  B Geiger
Journal:  Cell       Date:  1979-09       Impact factor: 41.582

5.  A vinculin-containing cortical lattice in skeletal muscle: transverse lattice elements ("costameres") mark sites of attachment between myofibrils and sarcolemma.

Authors:  J V Pardo; J D Siliciano; S W Craig
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

6.  Metavinculin and vinculin from mammalian smooth muscle: bulk isolation and characterization.

Authors:  M Gimona; D O Fürst; J V Small
Journal:  J Muscle Res Cell Motil       Date:  1987-08       Impact factor: 2.698

7.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

8.  Properties of smooth muscle vinculin.

Authors:  R R Evans; R M Robson; M H Stromer
Journal:  J Biol Chem       Date:  1984-03-25       Impact factor: 5.157

9.  Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes.

Authors:  B Geiger; K T Tokuyasu; A H Dutton; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

10.  Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts.

Authors:  W T Chen; S J Singer
Journal:  J Cell Biol       Date:  1982-10       Impact factor: 10.539
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