Literature DB >> 2125818

Preservation of ruminal bacterium capsules by using lysine in the electron microscopy fixative.

D E Akin1, L L Rigsby.   

Abstract

Ruminal bacteria from axenic cultures of Ruminococcus flavefaciens FD1, Butyrivibrio fibrisolvens 49, and bacterial types from the ruminal ecosystem that were fixed with 50 mM lysine (l-lysine hydrochloride) added to glutaraldehyde had better-preserved capsules and extracellular material than bacteria fixed without lysine.

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Year:  1990        PMID： 2125818      PMCID： PMC184870          DOI： 10.1128/aem.56.9.2933-2935.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Rumen bacterial interrelationships with plant tissue during degradation revealed by transmission electron microscopy.

Authors:  D E Akin; D Burdick; G E Michaels
Journal:  Appl Microbiol       Date:  1974-06

2.  Effect of 3-Phenylpropanoic Acid on Capsule and Cellulases of Ruminococcus albus 8.

Authors:  R J Stack; R E Hungate
Journal:  Appl Environ Microbiol       Date:  1984-07       Impact factor: 4.792

3.  Ultrastructure of rumen bacterial attachment to forage cell walls.

Authors:  D E Akin
Journal:  Appl Environ Microbiol       Date:  1976-04       Impact factor: 4.792

4.  Improved preservation of bacterial capsule for electron microscopy.

Authors:  M Jacques; L Graham
Journal:  J Electron Microsc Tech       Date:  1989-02

5.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

6.  Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls.

Authors:  D E Akin
Journal:  Appl Environ Microbiol       Date:  1980-01       Impact factor: 4.792

7.  Ultrastructure of cell envelopes of bacteria of the bovine rumen.

Authors:  K J Cheng; J W Costerton
Journal:  Appl Microbiol       Date:  1975-06

8.  A new fixative for the preservation of actin filaments: fixation of pure actin filament pellets.

Authors:  J Boyles; L Anderson; P Hutcherson
Journal:  J Histochem Cytochem       Date:  1985-11       Impact factor: 2.479

9.  Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium.

Authors:  K J Cheng; J W Costerton
Journal:  J Bacteriol       Date:  1977-03       Impact factor: 3.490

10.  Adhesion of Bacteroides succinogenes in pure culture and in the presence of Ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (Lolium perenne).

Authors:  M J Latham; B E Brooker; G L Pettipher; P J Harris
Journal:  Appl Environ Microbiol       Date:  1978-06       Impact factor: 4.792
  10 in total

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