AIMS: To investigate the effect of medium compositions and culture conditions on keratinase production by a novel thermophilic fungus Myceliophthora thermophila (Apinis) Oorschot strain GZUIFR-H49-1. METHODS AND RESULTS: The thermophilic strain GZUIFR-H49-1 with keratinolytic ability was characterized and identified as a strain of M. thermophila on the basis of its morphological characters and molecular analysis of ITS1-5.8S-ITS2 rDNA sequence. Among the medium compositions tested, the soluble starch (SS), urea, sodium thiosulfate and CaCl2 were the most effective C-source, N-source, S-source and mineral ion, respectively, by employing the single-factor experiment. The urea and pH value were the significant factors (P < 0·05) for the keratinase production in this experiment condition using Plackett–Burman factorial design. The conditions of keratinase production were further optimized by Box–Behnken design. Consequently, there was a 6·4-fold increase (5100 U l−1) in the keratinase activity than the initial value (800 U l−1) by this optimal process. CONCLUSIONS: This study indicated that the optimization design proved a useful and powerful tool for the development of optimal medium compositions and culture conditions. Myceliophthora thermophila strain GZUIFR-H49-1 was a promising fungus strain for keratinase production.
AIMS: To investigate the effect of medium compositions and culture conditions on keratinase production by a novel thermophilic fungus Myceliophthora thermophila (Apinis) Oorschot strain GZUIFR-H49-1. METHODS AND RESULTS: The thermophilic strain GZUIFR-H49-1 with keratinolytic ability was characterized and identified as a strain of M. thermophila on the basis of its morphological characters and molecular analysis of ITS1-5.8S-ITS2 rDNA sequence. Among the medium compositions tested, the soluble starch (SS), urea, sodium thiosulfate and CaCl2 were the most effective C-source, N-source, S-source and mineral ion, respectively, by employing the single-factor experiment. The urea and pH value were the significant factors (P < 0·05) for the keratinase production in this experiment condition using Plackett–Burman factorial design. The conditions of keratinase production were further optimized by Box–Behnken design. Consequently, there was a 6·4-fold increase (5100 U l−1) in the keratinase activity than the initial value (800 U l−1) by this optimal process. CONCLUSIONS: This study indicated that the optimization design proved a useful and powerful tool for the development of optimal medium compositions and culture conditions. Myceliophthora thermophila strain GZUIFR-H49-1 was a promising fungus strain for keratinase production.
Authors: Joost van den Brink; Gonny C J van Muiswinkel; Bart Theelen; Sandra W A Hinz; Ronald P de Vries Journal: Appl Environ Microbiol Date: 2012-12-14 Impact factor: 4.792
Authors: Ana Maria Mazotto; Sabrina M L Cedrola; Edilma P de Souza; Sonia Couri; Alane B Vermelho Journal: 3 Biotech Date: 2022-03-11 Impact factor: 2.406
Authors: Ana Maria Mazotto; Rosalie Reed Rodrigues Coelho; Sabrina Martins Lage Cedrola; Marcos Fábio de Lima; Sonia Couri; Edilma Paraguai de Souza; Alane Beatriz Vermelho Journal: Enzyme Res Date: 2011-08-01