INTRODUCTION: MicroRNAs (miRNAs) are a class of small (19-25 nucleotides) noncoding RNAs that regulate the expressions of a wide variety of genes, including some involved in cancer development. Some recent studies show that DNA methylation contributes to down-regulation of microRNA-137 (miR-137) during tumorigenesis. Whether down-regulation of miR-137 also exists in gastric cancer is unknown. AIM: Our aim was to test the hypothesis that down-regulation of miR-137 also exists in gastric cancer. METHODS: Expression of levels of miR-137 were examined using real-time PCR on paired gastric cancer and adjacent non-cancerous tissues. The methylation status is detected by MSP. RESULTS: Results show that miR-137 is downregulated by hypermethylation of the promoter in gastric cancer tissues. Epigenetic silencing of miR-137 induced an up-regulation of its targets, Cdc42. Restoration of the miR-137 expression in gastric cancer cell lines downregulated the Cdc42 expression. Restoration of the miR-137 and inactivation of Cdc42 induce apoptosis and cell cycle G1 arrest in gastric cancer cells. Furthermore, the miR-137 expression was found to be inversely correlated with CDC42 expression in gastric caner. CONCLUSIONS: miR-137 is frequently down-regulated in gastric cancer and is a negative regulator of Cdc42.
INTRODUCTION: MicroRNAs (miRNAs) are a class of small (19-25 nucleotides) noncoding RNAs that regulate the expressions of a wide variety of genes, including some involved in cancer development. Some recent studies show that DNA methylation contributes to down-regulation of microRNA-137 (miR-137) during tumorigenesis. Whether down-regulation of miR-137 also exists in gastric cancer is unknown. AIM: Our aim was to test the hypothesis that down-regulation of miR-137 also exists in gastric cancer. METHODS: Expression of levels of miR-137 were examined using real-time PCR on paired gastric cancer and adjacent non-cancerous tissues. The methylation status is detected by MSP. RESULTS: Results show that miR-137 is downregulated by hypermethylation of the promoter in gastric cancer tissues. Epigenetic silencing of miR-137 induced an up-regulation of its targets, Cdc42. Restoration of the miR-137 expression in gastric cancer cell lines downregulated the Cdc42 expression. Restoration of the miR-137 and inactivation of Cdc42 induce apoptosis and cell cycle G1 arrest in gastric cancer cells. Furthermore, the miR-137 expression was found to be inversely correlated with CDC42 expression in gastric caner. CONCLUSIONS:miR-137 is frequently down-regulated in gastric cancer and is a negative regulator of Cdc42.
Authors: Ming Liu; Nan Lang; Meng Qiu; Feng Xu; Qiu Li; Qiulin Tang; Ji Chen; Xi Chen; Siyuan Zhang; Zhen Liu; Jitao Zhou; Yajie Zhu; Yu Deng; Yi Zheng; Feng Bi Journal: Int J Cancer Date: 2011-03-15 Impact factor: 7.396
Authors: Francesc Balaguer; Alexander Link; Juan Jose Lozano; Miriam Cuatrecasas; Takeshi Nagasaka; C Richard Boland; Ajay Goel Journal: Cancer Res Date: 2010-08-03 Impact factor: 12.701
Authors: Yi-Wen Huang; Joseph C Liu; Daniel E Deatherage; Jingqin Luo; David G Mutch; Paul J Goodfellow; David S Miller; Tim H-M Huang Journal: Cancer Res Date: 2009-11-03 Impact factor: 12.701
Authors: Yanming Feng; Sean M Hartig; John E Bechill; Elisabeth G Blanchard; Eva Caudell; Seth J Corey Journal: J Biol Chem Date: 2009-11-17 Impact factor: 5.157
Authors: Teresa Gómez Del Pulgar; Fátima Valdés-Mora; Eva Bandrés; Rosa Pérez-Palacios; Carolina Espina; Paloma Cejas; Miguel Angel García-Cabezas; Manuel Nistal; Enrique Casado; Manuel González-Barón; Jesús García-Foncillas; Juan Carlos Lacal Journal: Int J Oncol Date: 2008-07 Impact factor: 5.650
Authors: Lars Dyrskjøt; Marie S Ostenfeld; Jesper B Bramsen; Asli N Silahtaroglu; Philippe Lamy; Ramshanker Ramanathan; Niels Fristrup; Jens L Jensen; Claus L Andersen; Karsten Zieger; Sakari Kauppinen; Benedicte P Ulhøi; Jørgen Kjems; Michael Borre; Torben F Orntoft Journal: Cancer Res Date: 2009-06-01 Impact factor: 12.701