Literature DB >> 2121133

The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.

I Pilz1, E Schwarz, D G Kilburn, R C Miller, R A Warren, N R Gilkes.   

Abstract

CenA from Cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. X-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and cellulose-binding domains comprise the head and tail respectively. It appears that this structural and functional organization is common to several cellulases from bacteria and fungi.

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Year:  1990        PMID： 2121133      PMCID： PMC1149546          DOI： 10.1042/bj2710277

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis.

Authors:  P Tomme; H Van Tilbeurgh; G Pettersson; J Van Damme; J Vandekerckhove; J Knowles; T Teeri; M Claeyssens
Journal:  Eur J Biochem       Date:  1988-01-04

2.  Domain structure of cellobiohydrolase II as studied by small angle X-ray scattering: close resemblance to cellobiohydrolase I.

Authors:  P M Abuja; I Pilz; M Claeyssens; P Tomme
Journal:  Biochem Biophys Res Commun       Date:  1988-10-14       Impact factor: 3.575

3.  Measurement of protein by spectrophotometry at 205 nm.

Authors:  R K Scopes
Journal:  Anal Biochem       Date:  1974-05       Impact factor: 3.365

4.  Small-angle X-ray scattering.

Authors:  I Pilz; O Glatter; O Kratky
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

5.  Mode of action and substrate specificities of cellulases from cloned bacterial genes.

Authors:  N R Gilkes; M L Langsford; D G Kilburn; R C Miller; R A Warren
Journal:  J Biol Chem       Date:  1984-08-25       Impact factor: 5.157

6.  Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from Cellulomonas fimi.

Authors:  R A Warren; C F Beck; N R Gilkes; D G Kilburn; M L Langsford; R C Miller; G P O'Neill; M Scheufens; W K Wong
Journal:  Proteins       Date:  1986-12

7.  Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis.

Authors:  N R Gilkes; R A Warren; R C Miller; D G Kilburn
Journal:  J Biol Chem       Date:  1988-07-25       Impact factor: 5.157

8.  The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa.

Authors:  J Hall; H J Gilbert
Journal:  Mol Gen Genet       Date:  1988-07

9.  Structural and functional analysis of a bacterial cellulase by proteolysis.

Authors:  N R Gilkes; D G Kilburn; R C Miller; R A Warren
Journal:  J Biol Chem       Date:  1989-10-25       Impact factor: 5.157

10.  Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains.

Authors:  M L Langsford; N R Gilkes; B Singh; B Moser; R C Miller; R A Warren; D G Kilburn
Journal:  FEBS Lett       Date:  1987-12-10       Impact factor: 4.124
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  5 in total

1.  Low-resolution structures of proteins in solution retrieved from X-ray scattering with a genetic algorithm.

Authors:  P Chacón; F Morán; J F Díaz; E Pantos; J M Andreu
Journal:  Biophys J       Date:  1998-06       Impact factor: 4.033

2.  Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases.

Authors:  K M Kleman-Leyer; N R Gilkes; R C Miller; T K Kirk
Journal:  Biochem J       Date:  1994-09-01       Impact factor: 3.857

3.  Aspergillus niger β-glucosidase has a cellulase-like tadpole molecular shape: insights into glycoside hydrolase family 3 (GH3) β-glucosidase structure and function.

Authors:  Marisa A Lima; Mario Oliveira-Neto; Marco Antonio S Kadowaki; Flavio R Rosseto; Erica T Prates; Fabio M Squina; Adriana F P Leme; Munir S Skaf; Igor Polikarpov
Journal:  J Biol Chem       Date:  2013-09-24       Impact factor: 5.157

4.  Structure, dynamics, and specificity of endoglucanase D from Clostridium cellulovorans.

Authors:  Christopher M Bianchetti; Phillip Brumm; Robert W Smith; Kevin Dyer; Greg L Hura; Thomas J Rutkoski; George N Phillips
Journal:  J Mol Biol       Date:  2013-06-08       Impact factor: 5.469

5.  Characterization of endoglucanase A from Clostridium cellulolyticum.

Authors:  H P Fierobe; C Gaudin; A Belaich; M Loutfi; E Faure; C Bagnara; D Baty; J P Belaich
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490
  5 in total

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