BACKGROUND: Clade C is the predominant HIV-1 strain infecting people in sub-Saharan Africa, India, and China and there is a critical need for a vaccine targeted to these areas. In this study we tested a DNA based vaccine that encodes the SIVgag, SIVpol and HIV-1 envelope clade C. METHODS: Rhesus macaques were immunized by electroporation with the DNA plasmid encoding optimized SIVgag, SIVpol and an HIV-1 env clade C with or without the adjuvant RANTES. Animals were monitored for immune responses and challenged following the final immunization with 25 animal infectious doses (AID) of SHIV-1157ipd3N4. RESULTS: We found that the vaccine induced high levels of antigen specific IFN-γ producing effector cells and the capacity for CD4+ and CD8+ to proliferate upon antigen stimulation. Importantly, we found that the vaccine induced antibody titers as high as 1/4000. These antibodies were capable of neutralizing tier 1 HIV-1 viruses. Finally, when macaques were challenged with SHIV, viral loads were controlled in vaccinated groups. CONCLUSION: We conclude that immunization with a simian/human immunodeficiency virus DNA-based vaccine delivered by electroporation can induce cellular and humoral immune responses that are able to control viral replication.
BACKGROUND: Clade C is the predominant HIV-1 strain infecting people in sub-Saharan Africa, India, and China and there is a critical need for a vaccine targeted to these areas. In this study we tested a DNA based vaccine that encodes the SIVgag, SIVpol and HIV-1 envelope clade C. METHODS:Rhesus macaques were immunized by electroporation with the DNA plasmid encoding optimized SIVgag, SIVpol and an HIV-1 env clade C with or without the adjuvant RANTES. Animals were monitored for immune responses and challenged following the final immunization with 25 animal infectious doses (AID) of SHIV-1157ipd3N4. RESULTS: We found that the vaccine induced high levels of antigen specific IFN-γ producing effector cells and the capacity for CD4+ and CD8+ to proliferate upon antigen stimulation. Importantly, we found that the vaccine induced antibody titers as high as 1/4000. These antibodies were capable of neutralizing tier 1 HIV-1 viruses. Finally, when macaques were challenged with SHIV, viral loads were controlled in vaccinated groups. CONCLUSION: We conclude that immunization with a simian/human immunodeficiency virus DNA-based vaccine delivered by electroporation can induce cellular and humoral immune responses that are able to control viral replication.
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Authors: Viraj Kulkarni; Margherita Rosati; Antonio Valentin; Rashmi Jalah; Candido Alicea; Lei Yu; Yongjun Guan; Xiaoying Shen; Georgia D Tomaras; Celia LaBranche; David C Montefiori; Carmela Irene; Rajasekhar Prattipati; Abraham Pinter; Sean M Sullivan; George N Pavlakis; Barbara K Felber Journal: Hum Vaccin Immunother Date: 2013-07-02 Impact factor: 3.452
Authors: Josephine H Cox; Maria G Ferrari; Patricia Earl; James R Lane; Linda L Jagodzinski; Victoria R Polonis; Ellen G Kuta; Jean D Boyer; Silvia Ratto-Kim; Leigh-Anne Eller; Doan-Trang Pham; Lydia Hart; David Montefiori; Guido Ferrari; Stephanie Parrish; David B Weiner; Bernard Moss; Jerome H Kim; Deborah Birx; Thomas C VanCott Journal: Vaccine Date: 2012-01-09 Impact factor: 3.641
Authors: Peter Hayes; Jill Gilmour; Andrea von Lieven; Dilbinder Gill; Lorna Clark; Jakub Kopycinski; Hannah Cheeseman; Amy Chung; Galit Alter; Len Dally; Devika Zachariah; Angela Lombardo; James Ackland; Eddy Sayeed; Akil Jackson; Marta Boffito; Brian Gazzard; Patricia E Fast; Josephine H Cox; Dagna Laufer Journal: Clin Vaccine Immunol Date: 2013-01-23