BACKGROUND: Amyloid peptides are established biomarkers for Alzheimer's disease but their analysis presents major challenges. RESULTS: In this article, we describe the use of ultra high-performance LC and API4000 triple quadrupole instrumentation for the quantification of amyloid peptides (Aβ(1-38) and Aβ(1-42)) in cerebrospinal fluid (CSF), using electrospray ionization with negative ion multiple reaction monitoring transitions. Sample preparation was simplified by the addition of acetonitrile and ammonium hydroxide. Although excellent sensitivity and precision was demonstrated, we observed differences in matrix suppression effects when using artificial CSF versus canine CSF for calibration curves and quality control samples. CONCLUSION: A case study shows that the method can be used to determine the relative levels of two key peptides (Aβ(1-38) and Aβ(1-42)) compared with their predose values (a screening assay) in support of preclinical studies.
BACKGROUND: Amyloid peptides are established biomarkers for Alzheimer's disease but their analysis presents major challenges. RESULTS: In this article, we describe the use of ultra high-performance LC and API4000 triple quadrupole instrumentation for the quantification of amyloid peptides (Aβ(1-38) and Aβ(1-42)) in cerebrospinal fluid (CSF), using electrospray ionization with negative ion multiple reaction monitoring transitions. Sample preparation was simplified by the addition of acetonitrile and ammonium hydroxide. Although excellent sensitivity and precision was demonstrated, we observed differences in matrix suppression effects when using artificial CSF versus canine CSF for calibration curves and quality control samples. CONCLUSION: A case study shows that the method can be used to determine the relative levels of two key peptides (Aβ(1-38) and Aβ(1-42)) compared with their predose values (a screening assay) in support of preclinical studies.
Authors: Antoine Leuzy; Konstantinos Chiotis; Steen G Hasselbalch; Juha O Rinne; Alexandre de Mendonça; Markus Otto; Alberto Lleó; Miguel Castelo-Branco; Isabel Santana; Jarkko Johansson; Sarah Anderl-Straub; Christine A F von Arnim; Ambros Beer; Rafael Blesa; Juan Fortea; Sanna-Kaisa Herukka; Erik Portelius; Josef Pannee; Henrik Zetterberg; Kaj Blennow; Agneta Nordberg Journal: Brain Date: 2016-07-07 Impact factor: 13.501
Authors: Jens Rose Fogh; Anne-Marie Jacobsen; Tam T T N Nguyen; Kasper D Rand; Line Rørbæk Olsen Journal: Anal Bioanal Chem Date: 2020-02-01 Impact factor: 4.142