| Literature DB >> 21174981 |
Qing Hu1, Li Li, Yanjuan Wang, Wenjuan Zhao, Hongyan Qi, Guoqiang Zhuang.
Abstract
The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome of Escherichia coli DH5alpha, and phiYFP was introduced into E. coli DH5alpha as a reporter gene to construct an arsenic-resistant whole-cell biosensor (WCB-11) in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression of phiYFP. When strain WCB-11 was exposed to As3+ and As5+, the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field.Entities:
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Year: 2010 PMID： 21174981 DOI： 10.1016/s1001-0742(09)60277-1
Source DB: PubMed Journal: J Environ Sci (China) ISSN： 1001-0742 Impact factor: 5.565