Literature DB >> 21172389

Abbott RealTime HIV-1 m2000rt viral load testing: manual extraction versus the automated m2000sp extraction.

Lesley E Scott1, John A Crump, Emma Msuya, Anne B Morrissey, Willem F Venter, Wendy S Stevens.   

Abstract

The Abbott RealTime HIV-1 assay is a real-time nucleic acid amplification assay available for HIV-1 viral load quantitation. The assay has a platform for automated extraction of viral RNA from plasma or dried blood spot samples, and an amplification platform with real time fluorescent detection. Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID: 21172389      PMCID: PMC3164890          DOI: 10.1016/j.jviromet.2010.12.006

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  7 in total

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Authors:  Lesley E Scott; Jacky S Galpin; Deborah K Glencross
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2.  Performance of the new Roche Cobas AmpliPrep-Cobas TaqMan version 2.0 human immunodeficiency virus type 1 assay.

Authors:  Lesley Scott; Sergio Carmona; Wendy Stevens
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3.  Comparing methods of measurement: why plotting difference against standard method is misleading.

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Journal:  Lancet       Date:  1995-10-21       Impact factor: 79.321

Review 4.  Diagnosis of HIV infection and laboratory monitoring of its therapy.

Authors:  G Yilmaz
Journal:  J Clin Virol       Date:  2001-06       Impact factor: 3.168

5.  Evaluation of the Abbott m2000 RealTime human immunodeficiency virus type 1 (HIV-1) assay for HIV load monitoring in South Africa compared to the Roche Cobas AmpliPrep-Cobas Amplicor, Roche Cobas AmpliPrep-Cobas TaqMan HIV-1, and BioMerieux NucliSENS EasyQ HIV-1 assays.

Authors:  Lesley E Scott; Lara D Noble; Jackie Moloi; Linda Erasmus; Willem D F Venter; Wendy Stevens
Journal:  J Clin Microbiol       Date:  2009-05-06       Impact factor: 5.948

6.  Evaluation of the Abbott m2000rt RealTime HIV-1 assay with manual sample preparation compared with the ROCHE COBAS AmpliPrep/AMPLICOR HIV-1 MONITOR v1.5 using specimens from East Africa.

Authors:  John A Crump; Lesley E Scott; Emma Msuya; Anne B Morrissey; Ekyafyose E Kimaro; John F Shao; Wendy S Stevens
Journal:  J Virol Methods       Date:  2009-09-01       Impact factor: 2.014

7.  Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR.

Authors:  Shihai Huang; John Salituro; Ning Tang; Ka-Cheung Luk; John Hackett; Priscilla Swanson; Gavin Cloherty; Wai-Bing Mak; John Robinson; Klara Abravaya
Journal:  Nucleic Acids Res       Date:  2007-08-09       Impact factor: 16.971

  7 in total
  11 in total

1.  Q fever, spotted fever group, and typhus group rickettsioses among hospitalized febrile patients in northern Tanzania.

Authors:  Malavika Prabhu; William L Nicholson; Aubree J Roche; Gilbert J Kersh; Kelly A Fitzpatrick; Lindsay D Oliver; Robert F Massung; Anne B Morrissey; John A Bartlett; Jecinta J Onyango; Venance P Maro; Grace D Kinabo; Wilbrod Saganda; John A Crump
Journal:  Clin Infect Dis       Date:  2011-08       Impact factor: 9.079

2.  Leptospirosis among hospitalized febrile patients in northern Tanzania.

Authors:  Holly M Biggs; Duy M Bui; Renee L Galloway; Robyn A Stoddard; Sean V Shadomy; Anne B Morrissey; John A Bartlett; Jecinta J Onyango; Venance P Maro; Grace D Kinabo; Wilbrod Saganda; John A Crump
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3.  Initiation of antiretroviral therapy in HIV-infected adults with skin complaints in northern Tanzania.

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4.  Predicting mortality for paediatric inpatients where malaria is uncommon.

Authors:  Dana C Clifton; Habib O Ramadhani; Levina J Msuya; Boniface N Njau; Grace D Kinabo; Ann M Buchanan; John A Crump
Journal:  Arch Dis Child       Date:  2012-08-07       Impact factor: 3.791

5.  Chikungunya and dengue fever among hospitalized febrile patients in northern Tanzania.

Authors:  Julian T Hertz; O Michael Munishi; Eng Eong Ooi; Shiqin Howe; Wen Yan Lim; Angelia Chow; Anne B Morrissey; John A Bartlett; Jecinta J Onyango; Venance P Maro; Grace D Kinabo; Wilbrod Saganda; Duane J Gubler; John A Crump
Journal:  Am J Trop Med Hyg       Date:  2012-01       Impact factor: 2.345

6.  Invasive bacterial and fungal infections among hospitalized HIV-infected and HIV-uninfected children and infants in northern Tanzania.

Authors:  John A Crump; Habib O Ramadhani; Anne B Morrissey; Levina J Msuya; Lan-Yan Yang; Shein-Chung Chow; Susan C Morpeth; Hugh Reyburn; Boniface N Njau; Andrea V Shaw; Helmut C Diefenthal; John A Bartlett; John F Shao; Werner Schimana; Coleen K Cunningham; Grace D Kinabo
Journal:  Trop Med Int Health       Date:  2011-04-07       Impact factor: 2.622

7.  Brucellosis among hospitalized febrile patients in northern Tanzania.

Authors:  Andrew J Bouley; Holly M Biggs; Robyn A Stoddard; Anne B Morrissey; John A Bartlett; Isaac A Afwamba; Venance P Maro; Grace D Kinabo; Wilbrod Saganda; Sarah Cleaveland; John A Crump
Journal:  Am J Trop Med Hyg       Date:  2012-10-22       Impact factor: 2.345

8.  Utility of rapid antibody tests to exclude HIV-1 infection among infants and children aged <18 months in a low-resource setting.

Authors:  Ann M Buchanan; Behzad Nadjm; Ben Amos; George Mtove; David Sifuna; Coleen K Cunningham; John A Crump; Hugh Reyburn
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9.  Leptospirosis and human immunodeficiency virus co-infection among febrile inpatients in northern Tanzania.

Authors:  Holly M Biggs; Renee L Galloway; Duy M Bui; Annie B Morrissey; Venance P Maro; John A Crump
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10.  Etiology of severe non-malaria febrile illness in Northern Tanzania: a prospective cohort study.

Authors:  John A Crump; Anne B Morrissey; William L Nicholson; Robert F Massung; Robyn A Stoddard; Renee L Galloway; Eng Eong Ooi; Venance P Maro; Wilbrod Saganda; Grace D Kinabo; Charles Muiruri; John A Bartlett
Journal:  PLoS Negl Trop Dis       Date:  2013-07-18
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