Literature DB >> 21154549

Analysis of DNA replication in Saccharomyces cerevisiae by two-dimensional and pulsed-field gel electrophoresis.

Satoru Ide1, Takehiko Kobayashi.   

Abstract

Two methods to analyze DNA replication in budding yeast are described here. One is two-dimensional (2D) gel electrophoresis, which has been used to analyze replication intermediates in yeast. The other method is pulsed-field gel electrophoresis (PFGE) that monitors the replication status of a chromosome. These are convenient and easy, and they do not require specialized equipment.

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Year:  2010        PMID： 21154549     DOI： 10.1002/0471143030.cb2214s49

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  8 in total

1.  The Human RNA Polymerase I Transcription Terminator Complex Acts as a Replication Fork Barrier That Coordinates the Progress of Replication with rRNA Transcription Activity.

Authors:  Yufuko Akamatsu; Takehiko Kobayashi
Journal:  Mol Cell Biol       Date:  2015-03-16       Impact factor: 4.272

2.  High levels of histones promote whole-genome-duplications and trigger a Swe1WEE1-dependent phosphorylation of Cdc28CDK1.

Authors:  Douglas Maya Miles; Vincent Geli; Xenia Peñate; Trinidad Sanmartín Olmo; Frederic Jourquin; Maria Cruz Muñoz Centeno; Manuel Mendoza; Marie-Noelle Simon; Sebastian Chavez
Journal:  Elife       Date:  2018-03-27       Impact factor: 8.140

3.  Molecular basis of the essential s phase function of the rad53 checkpoint kinase.

Authors:  Nicolas C Hoch; Eric S-W Chen; Robert Buckland; Shun-Chung Wang; Alessandro Fazio; Andrew Hammet; Achille Pellicioli; Andrei Chabes; Ming-Daw Tsai; Jörg Heierhorst
Journal:  Mol Cell Biol       Date:  2013-06-10       Impact factor: 4.272

4.  The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.

Authors:  Shuai Lu; Kenneth K Lee; Bethany Harris; Bo Xiong; Tania Bose; Anita Saraf; Gaye Hattem; Laurence Florens; Chris Seidel; Jennifer L Gerton
Journal:  EMBO Rep       Date:  2014-03-14       Impact factor: 8.807

5.  Ribosomal RNA gene repeats associate with the nuclear pore complex for maintenance after DNA damage.

Authors:  Chihiro Horigome; Eri Unozawa; Takamasa Ooki; Takehiko Kobayashi
Journal:  PLoS Genet       Date:  2019-04-18       Impact factor: 5.917

6.  The CCR4-NOT Complex Maintains Stability and Transcription of rRNA Genes by Repressing Antisense Transcripts.

Authors:  Shun Hosoyamada; Mariko Sasaki; Takehiko Kobayashi
Journal:  Mol Cell Biol       Date:  2019-12-11       Impact factor: 4.272

7.  Rtt109 prevents hyper-amplification of ribosomal RNA genes through histone modification in budding yeast.

Authors:  Satoru Ide; Kimiko Saka; Takehiko Kobayashi
Journal:  PLoS Genet       Date:  2013-04-04       Impact factor: 5.917

Review 8.  Ribosomal RNA gene repeats, their stability and cellular senescence.

Authors:  Takehiko Kobayashi
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2014       Impact factor: 3.493
  8 in total

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