Literature DB >> 21151118

Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.

Leandro F Estrozi1, Daniel Boehringer, Shu-Ou Shan, Nenad Ban, Christiane Schaffitzel.   

Abstract

We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.

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Year:  2010        PMID： 21151118      PMCID： PMC3764645          DOI： 10.1038/nsmb.1952

Source DB:  PubMed          Journal:  Nat Struct Mol Biol        ISSN： 1545-9985            Impact factor:   15.369


  20 in total

1.  Crystal structure of the ribonucleoprotein core of the signal recognition particle.

Authors:  R T Batey; R P Rambo; L Lucast; B Rha; J A Doudna
Journal:  Science       Date:  2000-02-18       Impact factor: 47.728

2.  Important role of the tetraloop region of 4.5S RNA in SRP binding to its receptor FtsY.

Authors:  J R Jagath; N B Matassova; E de Leeuw; J M Warnecke; G Lentzen; M V Rodnina; J Luirink; W Wintermeyer
Journal:  RNA       Date:  2001-02       Impact factor: 4.942

3.  Substrate twinning activates the signal recognition particle and its receptor.

Authors:  Pascal F Egea; Shu-Ou Shan; Johanna Napetschnig; David F Savage; Peter Walter; Robert M Stroud
Journal:  Nature       Date:  2004-01-15       Impact factor: 49.962

4.  Heterodimeric GTPase core of the SRP targeting complex.

Authors:  Pamela J Focia; Irina V Shepotinovskaya; James A Seidler; Douglas M Freymann
Journal:  Science       Date:  2004-01-16       Impact factor: 47.728

Review 5.  Structure, function and evolution of the signal recognition particle.

Authors:  Kiyoshi Nagai; Chris Oubridge; Andreas Kuglstatter; Elena Menichelli; Catherine Isel; Luca Jovine
Journal:  EMBO J       Date:  2003-07-15       Impact factor: 11.598

Review 6.  Structural insights into the signal recognition particle.

Authors:  Jennifer A Doudna; Robert T Batey
Journal:  Annu Rev Biochem       Date:  2004       Impact factor: 23.643

7.  Structure of the conserved GTPase domain of the signal recognition particle.

Authors:  D M Freymann; R J Keenan; R M Stroud; P Walter
Journal:  Nature       Date:  1997-01-23       Impact factor: 49.962

8.  Crystal structure of the NG domain from the signal-recognition particle receptor FtsY.

Authors:  G Montoya; C Svensson; J Luirink; I Sinning
Journal:  Nature       Date:  1997-01-23       Impact factor: 49.962

Review 9.  Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.

Authors:  P Walter; A E Johnson
Journal:  Annu Rev Cell Biol       Date:  1994

10.  Requirement of GTP hydrolysis for dissociation of the signal recognition particle from its receptor.

Authors:  T Connolly; P J Rapiejko; R Gilmore
Journal:  Science       Date:  1991-05-24       Impact factor: 47.728
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  39 in total

1.  SIMIBI twins in protein targeting and localization.

Authors:  Gert Bange; Irmgard Sinning
Journal:  Nat Struct Mol Biol       Date:  2013-07       Impact factor: 15.369

2.  Direct visualization reveals dynamics of a transient intermediate during protein assembly.

Authors:  Xin Zhang; Vinh Q Lam; Yun Mou; Tetsunari Kimura; Jaeyoon Chung; Sowmya Chandrasekar; Jay R Winkler; Stephen L Mayo; Shu-ou Shan
Journal:  Proc Natl Acad Sci U S A       Date:  2011-04-04       Impact factor: 11.205

3.  Synergistic actions between the SRP RNA and translating ribosome allow efficient delivery of the correct cargos during cotranslational protein targeting.

Authors:  Kuang Shen; Xin Zhang; Shu-Ou Shan
Journal:  RNA       Date:  2011-04-01       Impact factor: 4.942

Review 4.  A tale of two GTPases in cotranslational protein targeting.

Authors:  Ishu Saraogi; David Akopian; Shu-Ou Shan
Journal:  Protein Sci       Date:  2011-09-27       Impact factor: 6.725

5.  Regulation by a chaperone improves substrate selectivity during cotranslational protein targeting.

Authors:  Aileen Ariosa; Jae Ho Lee; Shuai Wang; Ishu Saraogi; Shu-ou Shan
Journal:  Proc Natl Acad Sci U S A       Date:  2015-06-08       Impact factor: 11.205

6.  Molecular engineering strategies for visualizing low-affinity protein complexes.

Authors:  Qianqian Ming; David Gonzalez-Perez; Vincent C Luca
Journal:  Exp Biol Med (Maywood)       Date:  2019-06-11

7.  Co-evolution of Two GTPases Enables Efficient Protein Targeting in an RNA-less Chloroplast Signal Recognition Particle Pathway.

Authors:  Sowmya Chandrasekar; Michael J Sweredoski; Chang Ho Sohn; Sonja Hess; Shu-Ou Shan
Journal:  J Biol Chem       Date:  2016-11-28       Impact factor: 5.157

8.  Dynamic switch of the signal recognition particle from scanning to targeting.

Authors:  Wolf Holtkamp; Sejeong Lee; Thomas Bornemann; Tamara Senyushkina; Marina V Rodnina; Wolfgang Wintermeyer
Journal:  Nat Struct Mol Biol       Date:  2012-11-11       Impact factor: 15.369

Review 9.  Linkers in the structural biology of protein-protein interactions.

Authors:  Vishnu Priyanka Reddy Chichili; Veerendra Kumar; J Sivaraman
Journal:  Protein Sci       Date:  2013-01-08       Impact factor: 6.725

10.  The structural basis of FtsY recruitment and GTPase activation by SRP RNA.

Authors:  Felix Voigts-Hoffmann; Nikolaus Schmitz; Kuang Shen; Shu-Ou Shan; Sandro F Ataide; Nenad Ban
Journal:  Mol Cell       Date:  2013-11-07       Impact factor: 17.970
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