UNLABELLED: Angiogenesis is key to both the development and regeneration of the dentin-pulp complex. OBJECTIVE: We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events. METHODS: Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes. RESULTS: Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity. CONCLUSION: This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration.
UNLABELLED: Angiogenesis is key to both the development and regeneration of the dentin-pulp complex. OBJECTIVE: We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events. METHODS: Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes. RESULTS: Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity. CONCLUSION: This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration.
Authors: Avathamsa Athirasala; Anthony Tahayeri; Greeshma Thrivikraman; Cristiane M França; Nelson Monteiro; Victor Tran; Jack Ferracane; Luiz E Bertassoni Journal: Biofabrication Date: 2018-01-10 Impact factor: 9.954
Authors: N S Rodrigues; C M França; A Tahayeri; Z Ren; V P A Saboia; A J Smith; J L Ferracane; H Koo; L E Bertassoni Journal: J Dent Res Date: 2021-05-26 Impact factor: 8.924