Poliana Amanda Oliveira Silva1, Stella Maris de Freitas Lima2,3, Mirna de Souza Freire2,4, André Melro Murad5, Octávio Luiz Franco2,6, Taia Maria Berto Rezende7,8,9. 1. Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil. 2. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, room C-221, Brasília, Distrito Federal, 70.790-160, Brazil. 3. Curso de Odontologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil. 4. Curso de Odontologia, Centro Universitário do Planalto Central Aparecido dos Santos, UNICEPLAC, Brasília, Distrito Federal, Brazil. 5. Laboratório de Espectrometria de Massa, Embrapa Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brazil. 6. S-Inova Biotech, Pós-graduação em Biotecnologia , Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil. 7. Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil. taiambr@gmail.com. 8. Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, room C-221, Brasília, Distrito Federal, 70.790-160, Brazil. taiambr@gmail.com. 9. Curso de Odontologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil. taiambr@gmail.com.
Abstract
OBJECTIVES: The present study aimed to identify proteins obtained from pulp tissue and correlate with each clinical diagnosis (healthy pulp, inflamed pulp, and necrotic pulp). MATERIALS AND METHODS: A total of forty-five molars were used. Three biological replicas were evaluated. Lysis and sonication were used for protein extraction. Protein quantification was assessed by using the Bradford technique, and shotgun proteome analysis was performed by nanoUPLC-MSE using a Synapt G2 mass spectrometer. Mass spectra data were processed using the Waters PLGS software, and protein identification was done using the human Uniprot database appended to the PLGS search engine. RESULTS: A total of 123 different proteins were identified in all evaluated pulp conditions. Among these, 66 proteins were observed for healthy pulp, 66 for inflamed pulp, and 91 for necrotic pulp. Most protein identification was related to immune response, multi-organism process, platelet activation, and stress in inflamed pulp samples compared to healthy pulp. Proteins related to cellular component organization or biogenesis, developmental process, growth, immune response, multi-organism process, response to stimulus, signaling, stress, and transport were identified in cases of apical periodontitis compared to inflamed pulp. CONCLUSIONS: The progression of the disease to inflamed pulp promoted a high abundance of proteins related to the immune system and stress. Comparing the necrotic pulp with inflamed pulp conditions, a high abundance of proteins was noticed related to metabolism, transport, and response between organisms. CLINICAL RELEVANCE: This finding may assist in future studies of new markers, understanding of tissue engineering, and development of future products.
OBJECTIVES: The present study aimed to identify proteins obtained from pulp tissue and correlate with each clinical diagnosis (healthy pulp, inflamed pulp, and necrotic pulp). MATERIALS AND METHODS: A total of forty-five molars were used. Three biological replicas were evaluated. Lysis and sonication were used for protein extraction. Protein quantification was assessed by using the Bradford technique, and shotgun proteome analysis was performed by nanoUPLC-MSE using a Synapt G2 mass spectrometer. Mass spectra data were processed using the Waters PLGS software, and protein identification was done using the human Uniprot database appended to the PLGS search engine. RESULTS: A total of 123 different proteins were identified in all evaluated pulp conditions. Among these, 66 proteins were observed for healthy pulp, 66 for inflamed pulp, and 91 for necrotic pulp. Most protein identification was related to immune response, multi-organism process, platelet activation, and stress in inflamed pulp samples compared to healthy pulp. Proteins related to cellular component organization or biogenesis, developmental process, growth, immune response, multi-organism process, response to stimulus, signaling, stress, and transport were identified in cases of apical periodontitis compared to inflamed pulp. CONCLUSIONS: The progression of the disease to inflamed pulp promoted a high abundance of proteins related to the immune system and stress. Comparing the necrotic pulp with inflamed pulp conditions, a high abundance of proteins was noticed related to metabolism, transport, and response between organisms. CLINICAL RELEVANCE: This finding may assist in future studies of new markers, understanding of tissue engineering, and development of future products.
Authors: I A Mejàre; S Axelsson; T Davidson; F Frisk; M Hakeberg; T Kvist; A Norlund; A Petersson; I Portenier; H Sandberg; S Tranaeus; G Bergenholtz Journal: Int Endod J Date: 2012-02-13 Impact factor: 5.264
Authors: Taia M B Rezende; Stella M F Lima; Bernardo A Petriz; Osmar N Silva; Mirna S Freire; Octávio L Franco Journal: J Cell Physiol Date: 2013-12 Impact factor: 6.384
Authors: Jean-Christophe Farges; Brigitte Alliot-Licht; Emmanuelle Renard; Maxime Ducret; Alexis Gaudin; Anthony J Smith; Paul R Cooper Journal: Mediators Inflamm Date: 2015-10-11 Impact factor: 4.711