PURPOSE: Cervical cancer is the second most prevalent malignancy of women. Our aim was to identify additional marker protein patterns for objective diagnosis of squamous cervical cancer (SCC). EXPERIMENTAL DESIGN: Collected tissue biopsies of SCC, squamous vaginal cancer (SVC), normal cervical and vaginal mucosa were subjected to 2-DE, SameSpot analysis, MALDI-TOF-MS protein identification, and analysis of the expression of selected proteins by immunohistochemistry. RESULTS: In 148 protein spots selected by the difference in expression 99 proteins were identified. A differential protein pattern for SCC was, e.g. over-expressed (OE) eukaryotic translation initiation factor 3-2β, neutrophil cytosolic factor 2, annexin A6 (ANXA6), for SVC it was OE cathepsin D, γ-catenin, RAB2A, for both cancers it was OE apolipoprotein E, tropomyosin 3, HSPA8, and underexpressed cytokeratin 13, osteoglycin. In SCC nuclear expression of neutrophil cytosolic factor 2, PRDX2, HSP27 (nine of ten cases), ANXA6 (nine of ten cases) was observed while tropomyosin 4 was expressed only in two of ten cases. There was 81.1% (43/53) agreement between the expression of protein spots and the immune expression of proteins (www.proteinatlas.org). CONCLUSIONS AND CLINICAL RELEVANCE: SCC is characterized by specific tissue marker protein patterns that allow objective detection of the disease. They can become a basis for objective automated cytology-based screening and improve current diagnostics of SCC.
PURPOSE: Cervical cancer is the second most prevalent malignancy of women. Our aim was to identify additional marker protein patterns for objective diagnosis of squamous cervical cancer (SCC). EXPERIMENTAL DESIGN: Collected tissue biopsies of SCC, squamous vaginal cancer (SVC), normal cervical and vaginal mucosa were subjected to 2-DE, SameSpot analysis, MALDI-TOF-MS protein identification, and analysis of the expression of selected proteins by immunohistochemistry. RESULTS: In 148 protein spots selected by the difference in expression 99 proteins were identified. A differential protein pattern for SCC was, e.g. over-expressed (OE) eukaryotic translation initiation factor 3-2β, neutrophil cytosolic factor 2, annexin A6 (ANXA6), for SVC it was OE cathepsin D, γ-catenin, RAB2A, for both cancers it was OE apolipoprotein E, tropomyosin 3, HSPA8, and underexpressed cytokeratin 13, osteoglycin. In SCC nuclear expression of neutrophil cytosolic factor 2, PRDX2, HSP27 (nine of ten cases), ANXA6 (nine of ten cases) was observed while tropomyosin 4 was expressed only in two of ten cases. There was 81.1% (43/53) agreement between the expression of protein spots and the immune expression of proteins (www.proteinatlas.org). CONCLUSIONS AND CLINICAL RELEVANCE: SCC is characterized by specific tissue marker protein patterns that allow objective detection of the disease. They can become a basis for objective automated cytology-based screening and improve current diagnostics of SCC.
Authors: Rose M McConnell; Kalyani Inapudi; Naveen Kadasala; Karthika Yarlagadda; Priya Velusamy; Matthew S McConnell; Adam Green; Carol Trana; Kelley Sayyar; James S McConnell Journal: Med Chem Date: 2012-11 Impact factor: 2.745
Authors: M I Lomnytska; S Becker; I Bodin; A Olsson; K Hellman; A-C Hellström; M Mints; U Hellman; G Auer; S Andersson Journal: Br J Cancer Date: 2010-11-30 Impact factor: 7.640
Authors: Kai-Erik Uleberg; Ane Cecilie Munk; Cato Brede; Einar Gudlaugsson; Bianca van Diermen; Ivar Skaland; Anais Malpica; Emiel Am Janssen; Anne Hjelle; Jan Pa Baak Journal: Proteome Sci Date: 2011-06-28 Impact factor: 2.480
Authors: M I Lomnytska; S Becker; T Gemoll; C Lundgren; J Habermann; A Olsson; I Bodin; U Engström; U Hellman; K Hellman; A-C Hellström; S Andersson; M Mints; G Auer Journal: Br J Cancer Date: 2012-03-13 Impact factor: 7.640