Literature DB >> 2113383

Cloning of Clostridium cellulovorans endo-1,4-beta-glucanase genes.

O Shoseyov1, T Hamamoto, F Foong, R H Doi.   

Abstract

A Clostridium cellulovorans lambda gt11 gene bank was screened for endo-1,4-beta-glucanase [EC 3.2.1.4, EGase, Carboxy Methyl Cellulase (CMCase)] genes using a chromogenic substrate. Three genes (engA, engB, and engC) were isolated. The engB expressed the most active CMCase. The engA encoded a bifunctional enzyme that displayed endo-1,4-beta-glucanase and beta-glucosidase activities. The three recombinant glucanases, when expressed in Escherichia coli, were partially degraded into multiform active enzymes as evidenced by their SDS-PAGE-CMC zymograms. None of the clones could degrade crystalline cellulose, thus supporting the hypothesis that the integrity of the C. cellulovorans cellulase complex was essential for its 'true cellulase' activity.

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Year:  1990        PMID： 2113383     DOI： 10.1016/0006-291x(90)90382-w

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  A large gene cluster for the Clostridium cellulovorans cellulosome.

Authors:  Y Tamaru; S Karita; A Ibrahim; H Chan; R H Doi
Journal:  J Bacteriol       Date:  2000-10       Impact factor: 3.490

2.  Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A.

Authors:  O Shoseyov; M Takagi; M A Goldstein; R H Doi
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

3.  Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans.

Authors:  Koichiro Murashima; Akihiko Kosugi; Roy H Doi
Journal:  J Bacteriol       Date:  2005-10       Impact factor: 3.490

Review 4.  Carbohydrate binding modules: biochemical properties and novel applications.

Authors:  Oded Shoseyov; Ziv Shani; Ilan Levy
Journal:  Microbiol Mol Biol Rev       Date:  2006-06       Impact factor: 11.056

5.  Three surface layer homology domains at the N terminus of the Clostridium cellulovorans major cellulosomal subunit EngE.

Authors:  Y Tamaru; R H Doi
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

6.  Analysis of functional domains of endoglucanases from Clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction.

Authors:  T Hamamoto; F Foong; O Shoseyov; R H Doi
Journal:  Mol Gen Genet       Date:  1992-02

7.  Characterization and comparison of Clostridium cellulovorans endoglucanases-xylanases EngB and EngD hyperexpressed in Escherichia coli.

Authors:  F C Foong; R H Doi
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

8.  Outer membrane proteins of Fibrobacter succinogenes with potential roles in adhesion to cellulose and in cellulose digestion.

Authors:  Hyun-Sik Jun; Meng Qi; Joshua Gong; Emmanuel E Egbosimba; Cecil W Forsberg
Journal:  J Bacteriol       Date:  2007-07-20       Impact factor: 3.490

9.  Heterologous expression of endo-beta-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene.

Authors:  A Y Kim; G T Attwood; S M Holt; B A White; H P Blaschek
Journal:  Appl Environ Microbiol       Date:  1994-01       Impact factor: 4.792
  9 in total

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