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Literature DB >> 21131527

Cofactor trapping, a new method to produce flavin mononucleotide.

Ulrich Krauss¹, Vera Svensson, Astrid Wirtz, Esther Knieps-Grünhagen, Karl-Erich Jaeger.

Abstract

We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN.

Entities: Chemical Species

Mesh：

Substances：

Year: 2010 PMID： 21131527 PMCID： PMC3028748 DOI： 10.1128/AEM.01541-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

17 in total

1. Mutual exchange of kinetic properties by extended mutagenesis in two short LOV domain proteins from Pseudomonas putida.

Authors: Katrin Jentzsch; Astrid Wirtz; Franco Circolone; Thomas Drepper; Aba Losi; Wolfgang Gärtner; Karl-Erich Jaeger; Ulrich Krauss
Journal: Biochemistry Date: 2009-11-03 Impact factor: 3.162

2. Proposed steady-state kinetic mechanism for Corynebacterium ammoniagenes FAD synthetase produced by Escherichia coli.

Authors: I Efimov; V Kuusk; X Zhang; W S McIntire
Journal: Biochemistry Date: 1998-07-07 Impact factor: 3.162

Review 3. Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production.

Authors: K P Stahmann; J L Revuelta; H Seulberger
Journal: Appl Microbiol Biotechnol Date: 2000-05 Impact factor: 4.813

Review 4. In situ product removal as a tool for bioprocessing.

Authors: A Freeman; J M Woodley; M D Lilly
Journal: Biotechnology (N Y) Date: 1993-09

5. Regulation of riboflavin biosynthesis in Bacillus subtilis is affected by the activity of the flavokinase/flavin adenine dinucleotide synthetase encoded by ribC.

Authors: M Mack; A P van Loon; H P Hohmann
Journal: J Bacteriol Date: 1998-02 Impact factor: 3.490

6. An mRNA structure that controls gene expression by binding FMN.

Authors: Wade C Winkler; Smadar Cohen-Chalamish; Ronald R Breaker
Journal: Proc Natl Acad Sci U S A Date: 2002-11-27 Impact factor: 11.205

7. LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide.

Authors: J M Christie; M Salomon; K Nozue; M Wada; W R Briggs
Journal: Proc Natl Acad Sci U S A Date: 1999-07-20 Impact factor: 11.205

8. Regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation.

Authors: Alexey G Vitreschak; Dmitry A Rodionov; Andrey A Mironov; Mikhail S Gelfand
Journal: Nucleic Acids Res Date: 2002-07-15 Impact factor: 16.971

Cofactor trapping, a new method to produce flavin mononucleotide.

1. Mutual exchange of kinetic properties by extended mutagenesis in two short LOV domain proteins from Pseudomonas putida.

2. Proposed steady-state kinetic mechanism for Corynebacterium ammoniagenes FAD synthetase produced by Escherichia coli.

Review 3. Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production.

Review 4. In situ product removal as a tool for bioprocessing.

5. Regulation of riboflavin biosynthesis in Bacillus subtilis is affected by the activity of the flavokinase/flavin adenine dinucleotide synthetase encoded by ribC.

6. An mRNA structure that controls gene expression by binding FMN.

7. LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide.

8. Regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation.

9. The bacterial counterparts of plant phototropins.

Review 10. Riboflavin (vitamin B-2) and health.

1. YeeO from Escherichia coli exports flavins.

2. Characterization of flavin binding in oxygen-independent fluorescent reporters.

Review 3. Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.

Review 4. Production of riboflavin and related cofactors by biotechnological processes.