OBJECTIVE: To examine the effect of sperm morphology on embryo development at the chromosomal level. DESIGN: Prospective study. SETTING: Assisted fertilization center. PATIENT(S): Couples who underwent IVF-PGS cycle, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 60) or intracytoplasmic morphologically selected sperm injection (IMSI; n = 60). INTERVENTION(S): IVF in conjunction with preimplantation genetic screening (PGS). MAIN OUTCOME MEASURE(S): Sperm nuclear morphology at high-magnification ICSI and incidence of aneuploidy in derived embryo. RESULT(S): There was a significantly increased incidence for sex chromosome aneuploidy in ICSI embryos when compared with IMSI embryos (23.5% vs. 15.0%, respectively). High-magnification sperm selection was associated with a significantly lower risk of sex chromosome abnormalities (odds ratio [OR], 0.57; confidence interval [CI], 0.37-0.90). The incidence of chaotic embryos was also significantly higher with the ICSI procedure (27.5% vs. 18.8%), while the IMSI procedure was associated with a significantly lower risk of chaotic embryos (OR, 0.64; CI, 0.43-0.96). Moreover, the cycle cancellation rate was significantly higher in ICSI cycles (11.8% vs. 2.5%). High-magnification sperm selection was a significant predictor of the likelihood of cycle cancellation (OR, 0.26; CI, 0.11-0.62). CONCLUSION(S): Spermatozoa free of nuclear morphological malformations were found to be significantly associated with the lower incidence of aneuploidy in derived embryos, resulting in lower rates of cycle cancellation.
RCT Entities:
OBJECTIVE: To examine the effect of sperm morphology on embryo development at the chromosomal level. DESIGN: Prospective study. SETTING: Assisted fertilization center. PATIENT(S): Couples who underwent IVF-PGS cycle, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 60) or intracytoplasmic morphologically selected sperm injection (IMSI; n = 60). INTERVENTION(S): IVF in conjunction with preimplantation genetic screening (PGS). MAIN OUTCOME MEASURE(S): Sperm nuclear morphology at high-magnification ICSI and incidence of aneuploidy in derived embryo. RESULT(S): There was a significantly increased incidence for sex chromosome aneuploidy in ICSI embryos when compared with IMSI embryos (23.5% vs. 15.0%, respectively). High-magnification sperm selection was associated with a significantly lower risk of sex chromosome abnormalities (odds ratio [OR], 0.57; confidence interval [CI], 0.37-0.90). The incidence of chaotic embryos was also significantly higher with the ICSI procedure (27.5% vs. 18.8%), while the IMSI procedure was associated with a significantly lower risk of chaotic embryos (OR, 0.64; CI, 0.43-0.96). Moreover, the cycle cancellation rate was significantly higher in ICSI cycles (11.8% vs. 2.5%). High-magnification sperm selection was a significant predictor of the likelihood of cycle cancellation (OR, 0.26; CI, 0.11-0.62). CONCLUSION(S): Spermatozoa free of nuclear morphological malformations were found to be significantly associated with the lower incidence of aneuploidy in derived embryos, resulting in lower rates of cycle cancellation.
Authors: Martha Merino-Ruiz; Felipe Arturo Morales-Martínez; Edith Navar-Vizcarra; Otto H Valdés-Martínez; Luis H Sordia-Hernández; Donato Saldívar-Rodríguez; Oscar Vidal-Gutiérrez Journal: JBRA Assist Reprod Date: 2019-04-30
Authors: Danielle M Teixeira; Andre Hadyme Miyague; Mariana Ap Barbosa; Paula A Navarro; Nick Raine-Fenning; Carolina O Nastri; Wellington P Martins Journal: Cochrane Database Syst Rev Date: 2020-02-21
Authors: Daniel Luna; Roly Hilario; Julio Dueñas-Chacón; Rocío Romero; Patricia Zavala; Lucy Villegas; Javier García-Ferreyra Journal: Clin Med Insights Reprod Health Date: 2015-11-12