BACKGROUND: Elevated circulating cell-free DNA in plasma is reported in several critical diseases. This study hypothesized that since plasma nuclear and mitochondrial DNA substantially increase after acute ischemic stroke and decrease thereafter, their levels can predict treatment outcomes. METHODS: Plasma nuclear and mitochondrial DNA levels were serially examined in 50 acute ischemic stroke patients and in 50 at risk control subjects during the study period. RESULTS: Levels of plasma nuclear and mitochondrial DNA in patients with acute ischemic stroke were significantly higher than those in the controls (p<0.05). Elevated circulating nuclear DNA in plasma persisted until one month after the acute stroke. Levels of plasma nuclear DNA positively correlated to the clinical severity of stroke as reflected by the National Institutes of Health Stroke Scale. CONCLUSION: Levels of plasma nuclear and mitochondrial DNA reflect the severity of cerebral damage after acute cerebral infarction. Assay of plasma DNA levels can be considered a neuro-pathologic marker of patients with acute ischemic stroke. Copyright Â
BACKGROUND: Elevated circulating cell-free DNA in plasma is reported in several critical diseases. This study hypothesized that since plasma nuclear and mitochondrial DNA substantially increase after acute ischemic stroke and decrease thereafter, their levels can predict treatment outcomes. METHODS: Plasma nuclear and mitochondrial DNA levels were serially examined in 50 acute ischemic strokepatients and in 50 at risk control subjects during the study period. RESULTS: Levels of plasma nuclear and mitochondrial DNA in patients with acute ischemic stroke were significantly higher than those in the controls (p<0.05). Elevated circulating nuclear DNA in plasma persisted until one month after the acute stroke. Levels of plasma nuclear DNA positively correlated to the clinical severity of stroke as reflected by the National Institutes of Health Stroke Scale. CONCLUSION: Levels of plasma nuclear and mitochondrial DNA reflect the severity of cerebral damage after acute cerebral infarction. Assay of plasma DNA levels can be considered a neuro-pathologic marker of patients with acute ischemic stroke. Copyright Â
Authors: Charlotte Thålin; Melanie Demers; Bo Blomgren; Siu Ling Wong; Magnus von Arbin; Anders von Heijne; Ann Charlotte Laska; Håkan Wallén; Denisa D Wagner; Sara Aspberg Journal: Thromb Res Date: 2016-01-12 Impact factor: 3.944
Authors: Simon F De Meyer; Georgette L Suidan; Tobias A Fuchs; Marc Monestier; Denisa D Wagner Journal: Arterioscler Thromb Vasc Biol Date: 2012-05-24 Impact factor: 8.311
Authors: John S Harrington; Jin-Won Huh; Edward J Schenck; Kiichi Nakahira; Ilias I Siempos; Augustine M K Choi Journal: Chest Date: 2019-08-02 Impact factor: 9.410