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Literature DB >> 21125108

Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn2+/Mn2+) to monometallic (Zn2+-only) behavior.

Ying Xiao¹, Emily C Allen, Scott K Silverman.

Abstract

A deoxyribozyme that hydrolyzes DNA phosphodiester linkages with a requirement for both Zn(2+) and Mn(2+) is switched by only two nucleotide mutations to require Zn(2+) alone, demonstrating that DNA-catalyzed DNA hydrolysis can be achieved using only one metal ion cofactor.

Entities: Chemical Mutation Species

Mesh：

Substances：

Year: 2010 PMID： 21125108 PMCID： PMC3269124 DOI： 10.1039/c0cc04575f

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

24 in total

1. Functional compromises among pH tolerance, site specificity, and sequence tolerance for a DNA-hydrolyzing deoxyribozyme.

Authors: Ying Xiao; Madhavaiah Chandra; Scott K Silverman
Journal: Biochemistry Date: 2010-11-09 Impact factor: 3.162

2. Covalent Schiff base catalysis and turnover by a DNAzyme: a M2+ -independent AP-endonuclease mimic.

Authors: Jonathan P May; Richard Ting; Leonard Lermer; Jason M Thomas; Yoann Roupioz; David M Perrin
Journal: J Am Chem Soc Date: 2004-04-07 Impact factor: 15.419

3. A DNAzyme with three protein-like functional groups: enhancing catalytic efficiency of M2+-independent RNA cleavage.

Authors: Marcel Hollenstein; Christopher J Hipolito; Curtis H Lam; David M Perrin
Journal: Chembiochem Date: 2009-08-17 Impact factor: 3.164

4. Evidence for the metal-cofactor independence of an RNA phosphodiester-cleaving DNA enzyme.

Authors: C R Geyer; D Sen
Journal: Chem Biol Date: 1997-08

5. Bridging the gap between proteins and nucleic acids: a metal-independent RNAseA mimic with two protein-like functionalities.

Authors: D M Perrin; T Garestier; C Hélène
Journal: J Am Chem Soc Date: 2001-02-28 Impact factor: 15.419

6. Ribozyme-catalyzed dipeptide synthesis in monovalent metal ions alone.

Authors: Lele Sun; Zhiyong Cui; Chunfang Li; Shufang Huang; Biliang Zhang
Journal: Biochemistry Date: 2007-03-02 Impact factor: 3.162

7. Activity, folding and Z-DNA formation of the 8-17 DNAzyme in the presence of monovalent ions.

Authors: Debapriya Mazumdar; Nandini Nagraj; Hee-Kyung Kim; Xiangli Meng; Andrea K Brown; Qian Sun; Wei Li; Yi Lu
Journal: J Am Chem Soc Date: 2009-04-22 Impact factor: 15.419

8. The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone.

Authors: J B Murray; A A Seyhan; N G Walter; J M Burke; W G Scott
Journal: Chem Biol Date: 1998-10

9. DNA-catalyzed sequence-specific hydrolysis of DNA.

Authors: Madhavaiah Chandra; Amit Sachdeva; Scott K Silverman
Journal: Nat Chem Biol Date: 2009-08-16 Impact factor: 15.040

10. Sequence-specific cleavage of RNA in the absence of divalent metal ions by a DNAzyme incorporating imidazolyl and amino functionalities.

Authors: Alexander V Sidorov; Jane A Grasby; David M Williams
Journal: Nucleic Acids Res Date: 2004-03-05 Impact factor: 16.971

17 in total

Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn2+/Mn2+) to monometallic (Zn2+-only) behavior.

1. Functional compromises among pH tolerance, site specificity, and sequence tolerance for a DNA-hydrolyzing deoxyribozyme.

2. Covalent Schiff base catalysis and turnover by a DNAzyme: a M2+ -independent AP-endonuclease mimic.

3. A DNAzyme with three protein-like functional groups: enhancing catalytic efficiency of M2+-independent RNA cleavage.

4. Evidence for the metal-cofactor independence of an RNA phosphodiester-cleaving DNA enzyme.

5. Bridging the gap between proteins and nucleic acids: a metal-independent RNAseA mimic with two protein-like functionalities.

6. Ribozyme-catalyzed dipeptide synthesis in monovalent metal ions alone.

7. Activity, folding and Z-DNA formation of the 8-17 DNAzyme in the presence of monovalent ions.

8. The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone.

9. DNA-catalyzed sequence-specific hydrolysis of DNA.

10. Sequence-specific cleavage of RNA in the absence of divalent metal ions by a DNAzyme incorporating imidazolyl and amino functionalities.

1. DNA-catalyzed covalent modification of amino acid side chains in tethered and free peptide substrates.

2. DNA-catalyzed hydrolysis of esters and aromatic amides.

3. Pursuing DNA catalysts for protein modification.

4. DNA-catalyzed lysine side chain modification.

5. Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.

6. Biochemical and Biophysical Understanding of Metal Ion Selectivity of DNAzymes.

7. Lanthanide ions as required cofactors for DNA catalysts.

8. DNA catalysis of a normally disfavored RNA hydrolysis reaction.

9. Systematic evaluation of the dependence of deoxyribozyme catalysis on random region length.

10. Small, highly active DNAs that hydrolyze DNA.