| Literature DB >> 2111835 |
Abstract
A protocol for the rapid purification of ribonuclease T1 expressed from a chemically synthesized gene cloned into Escherichia coli is described. QAE ion-exchange and Sephadex G-50 chromatography are used to give over 300 mg (88% yield) of pure ribonuclease T1 from 61 of liquid culture in 3 days. We also report a new absorption coefficient for RNase T1: E1%278 nm = 15.4.Entities:
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Year: 1990 PMID： 2111835 DOI： 10.1016/0165-022x(90)90076-o
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X