BACKGROUND: Coumarins belong to an important group of useful drugs with diverse pharmacological properties. In the present study, the in vitro cytotoxicity of new coumarin-based benzopyranone derivatives containing diethylaminoethoxy (5), dimethylaminoethoxy (6), morpholinoethoxy (7), piperidinylethoxy (8) and pyrrolidinylethoxyl (9) amino side chain against human carcinoma (A549) and normal (LL47) lung cell lines was evaluated. MATERIALS AND METHODS: The cytotoxicity was evaluated by crystal violet dye binding assay. The effect of compound 9 on different phases of the cell cycle was determined using flow cytometry. RESULTS: In A549 cells, the 50% lethal dose (LD(50)) for compounds 5-9 were found to be 7.08, 5.0, 34.2, 8.33 and 5.83 μM, respectively, while in LL47 cells, the LD(50) values were found to be 16.7, 20.4, 34.6, 15.4 and 8.75 μM, respectively after 48 h treatment. Cell cycle data indicated that A549 cells were arrested at different phases depending on the concentration. CONCLUSION: Compounds 5-9 showed anticancer activity against lung cancer cell lines, while compound 6 showed highly selective anticancer activity.
BACKGROUND:Coumarins belong to an important group of useful drugs with diverse pharmacological properties. In the present study, the in vitro cytotoxicity of new coumarin-based benzopyranone derivatives containing diethylaminoethoxy (5), dimethylaminoethoxy (6), morpholinoethoxy (7), piperidinylethoxy (8) and pyrrolidinylethoxyl (9) amino side chain against humancarcinoma (A549) and normal (LL47) lung cell lines was evaluated. MATERIALS AND METHODS: The cytotoxicity was evaluated by crystal violet dye binding assay. The effect of compound 9 on different phases of the cell cycle was determined using flow cytometry. RESULTS: In A549 cells, the 50% lethal dose (LD(50)) for compounds 5-9 were found to be 7.08, 5.0, 34.2, 8.33 and 5.83 μM, respectively, while in LL47 cells, the LD(50) values were found to be 16.7, 20.4, 34.6, 15.4 and 8.75 μM, respectively after 48 h treatment. Cell cycle data indicated that A549 cells were arrested at different phases depending on the concentration. CONCLUSION: Compounds 5-9 showed anticancer activity against lung cancer cell lines, while compound 6 showed highly selective anticancer activity.
Authors: S Caltagirone; F O Ranelletti; A Rinelli; N Maggiano; A Colasante; P Musiani; F B Aiello; M Piantelli Journal: Am J Respir Cell Mol Biol Date: 1997-07 Impact factor: 6.914
Authors: Nareshkumar Jain; Jiayi Xu; Ramesh M Kanojia; Fuyong Du; Guo Jian-Zhong; Emmanuel Pacia; Muh-Tsann Lai; Amy Musto; George Allan; Michael Reuman; Xun Li; Dowon Hahn; Martin Cousineau; Sean Peng; David Ritchie; Ronald Russell; Scott Lundeen; Zhihua Sui Journal: J Med Chem Date: 2009-12-10 Impact factor: 7.446
Authors: Ramesh B Badisa; Selina F Darling-Reed; Patrick Joseph; John S Cooperwood; Lekan M Latinwo; Carl B Goodman Journal: Anticancer Res Date: 2009-08 Impact factor: 2.480
Authors: R D Thornes; L Daly; G Lynch; B Breslin; H Browne; H Y Browne; T Corrigan; P Daly; G Edwards; E Gaffney Journal: J Cancer Res Clin Oncol Date: 1994 Impact factor: 4.553
Authors: Musiliyu A Musa; Veera L D Badisa; Lekan M Latinwo; John Cooperwood; Andre Sinclair; Ahkinyala Abdullah Journal: Anticancer Res Date: 2011-06 Impact factor: 2.480
Authors: Lina A Al-Ani; Wageeh A Yehye; Farkaad A Kadir; Najihah M Hashim; Mohammed A AlSaadi; Nurhidayatullaili M Julkapli; Vincent K S Hsiao Journal: PLoS One Date: 2019-05-14 Impact factor: 3.240
Authors: Gamze Göger; Ümmühan Türkyolu; Ezgi Nur Gürşen; Süleyman Yur; Abdullah Burak Karaduman; Fatih Göger; Mehmet Tekin; Gülmira Özek Journal: Turk J Chem Date: 2021-04-28 Impact factor: 1.239