OBJECTIVE: Our recent microarray study detected decreases in the expression of phospholipase C beta 1 mRNA in the dorsolateral prefrontal cortex from subjects with schizophrenia at different stages of illness. Thus we aimed to validate and extend these findings. METHOD: We measured levels of mRNA and protein for phospholipase C beta 1 variant a and b using real-time PCR and western blot analysis, respectively, in the dorsolateral prefrontal cortex from subjects with schizophrenia, who had a short (< 7 years) or long (> 22 years) duration of illness. RESULTS: Compared to age/sex matched controls, levels of phospholipase C beta 1 variant a and b mRNAs were decreased (-33% and -50%, respectively) in short duration schizophrenia. By contrast, only variant a mRNA was decreased (-24%) in long duration schizophrenia. There was no significant difference in the protein levels of either phospholipase C beta 1 variant in schizophrenia, irrespective of duration of illness (variant a; P = 0.84, variant b; P = 0.73). CONCLUSION: Our data confirm that phospholipase C beta 1 transcript levels are decreased in the dorsolateral prefrontal cortex from subjects with schizophrenia. However, the changes in levels of mRNA do not translate into a change at the level of protein. It is possible protein expression is regulated independently of mRNA and it remains to be determined whether there is a functional consequence of this change in mRNA relating to the pathophysiology of schizophrenia.
OBJECTIVE: Our recent microarray study detected decreases in the expression of phospholipase C beta 1 mRNA in the dorsolateral prefrontal cortex from subjects with schizophrenia at different stages of illness. Thus we aimed to validate and extend these findings. METHOD: We measured levels of mRNA and protein for phospholipase C beta 1 variant a and b using real-time PCR and western blot analysis, respectively, in the dorsolateral prefrontal cortex from subjects with schizophrenia, who had a short (< 7 years) or long (> 22 years) duration of illness. RESULTS: Compared to age/sex matched controls, levels of phospholipase C beta 1 variant a and b mRNAs were decreased (-33% and -50%, respectively) in short duration schizophrenia. By contrast, only variant a mRNA was decreased (-24%) in long duration schizophrenia. There was no significant difference in the protein levels of either phospholipase C beta 1 variant in schizophrenia, irrespective of duration of illness (variant a; P = 0.84, variant b; P = 0.73). CONCLUSION: Our data confirm that phospholipase C beta 1 transcript levels are decreased in the dorsolateral prefrontal cortex from subjects with schizophrenia. However, the changes in levels of mRNA do not translate into a change at the level of protein. It is possible protein expression is regulated independently of mRNA and it remains to be determined whether there is a functional consequence of this change in mRNA relating to the pathophysiology of schizophrenia.
Authors: Natalia V Malkova; Joseph J Gallagher; Collin Z Yu; Russell E Jacobs; Paul H Patterson Journal: Proc Natl Acad Sci U S A Date: 2014-06-02 Impact factor: 11.205
Authors: C-Y Lai; S-Y Lee; E Scarr; Y-H Yu; Y-T Lin; C-M Liu; T-J Hwang; M H Hsieh; C-C Liu; Y-L Chien; M Udawela; A S Gibbons; I P Everall; H-G Hwu; B Dean; W J Chen Journal: Transl Psychiatry Date: 2016-01-19 Impact factor: 6.222
Authors: E Scarr; J M Craig; M J Cairns; M S Seo; J C Galati; N J Beveridge; A Gibbons; S Juzva; B Weinrich; M Parkinson-Bates; A P Carroll; R Saffery; B Dean Journal: Transl Psychiatry Date: 2013-02-19 Impact factor: 6.222
Authors: Elizabeth Scarr; Madhara Udawela; Mark A Greenough; Jaclyn Neo; Myoung Suk Seo; Tammie T Money; Aradhana Upadhyay; Ashley I Bush; Ian P Everall; Elizabeth A Thomas; Brian Dean Journal: NPJ Schizophr Date: 2016-03-09