Literature DB >> 2108907

Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells.

J H Camonis1, M Cassan, J P Rousset.   

Abstract

Gene expression in heterologous species is a powerful tool for the cloning and characterization of genes. Here, we present a family of expression shuttle vectors which work in the budding yeast, Saccharomyces cerevisiae, and also in mammalian cells. The quantity of product expressed by the gene under study can be modulated in yeast by virtue of the control over plasmid copy number and culture conditions.

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Year:  1990        PMID： 2108907     DOI： 10.1016/0378-1119(90)90288-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  13 in total

1.  ami1, an orthologue of the Aspergillus nidulans apsA gene, is involved in nuclear migration events throughout the life cycle of Podospora anserina.

Authors:  F Graïa; V Berteaux-Lecellier; D Zickler; M Picard
Journal:  Genetics       Date:  2000-06       Impact factor: 4.562

2.  The mating types of Podospora anserina: functional analysis and sequence of the fertilization domains.

Authors:  R Debuchy; E Coppin
Journal:  Mol Gen Genet       Date:  1992-05

3.  Fine-tuning of translation termination efficiency in Saccharomyces cerevisiae involves two factors in close proximity to the exit tunnel of the ribosome.

Authors:  Isabelle Hatin; Céline Fabret; Olivier Namy; Wayne A Decatur; Jean-Pierre Rousset
Journal:  Genetics       Date:  2007-05-04       Impact factor: 4.562

4.  A homologue of the yeast SHE4 gene is essential for the transition between the syncytial and cellular stages during sexual reproduction of the fungus Podospora anserina.

Authors:  V Berteaux-Lecellier; D Zickler; R Debuchy; A Panvier-Adoutte; C Thompson-Coffe; M Picard
Journal:  EMBO J       Date:  1998-08-10       Impact factor: 11.598

5.  Deletion of the mating-type sequences in Podospora anserina abolishes mating without affecting vegetative functions and sexual differentiation.

Authors:  E Coppin; S Arnaise; V Contamine; M Picard
Journal:  Mol Gen Genet       Date:  1993-11

6.  Two copies of mthmg1, encoding a novel mitochondrial HMG-like protein, delay accumulation of mitochondrial DNA deletions in Podospora anserina.

Authors:  Michelle Dequard-Chablat; Cynthia Allandt
Journal:  Eukaryot Cell       Date:  2002-08

7.  In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity.

Authors:  J P Tassan; K Le Guellec; M Kress; M Faure; J Camonis; M Jacquet; M Philippe
Journal:  Mol Cell Biol       Date:  1993-05       Impact factor: 4.272

8.  The C-terminal part of the CDC25 gene product has Ras-nucleotide exchange activity when present in a chimeric SDC25-CDC25 protein.

Authors:  E Boy-Marcotte; A Buu; C Soustelle; P Poullet; A Parmeggiani; M Jacquet
Journal:  Curr Genet       Date:  1993 May-Jun       Impact factor: 3.886

9.  Two subclasses of guanine exchange factor (GEF) domains revealed by comparison of activities of chimeric genes constructed from CDC25, SDC25 and BUD5 in Saccharomyces cerevisiae.

Authors:  C Camus; E Boy-Marcotte; M Jacquet
Journal:  Mol Gen Genet       Date:  1994-10-28

10.  Versatile vectors to study recoding: conservation of rules between yeast and mammalian cells.

Authors:  G Stahl; L Bidou; J P Rousset; M Cassan
Journal:  Nucleic Acids Res       Date:  1995-05-11       Impact factor: 16.971
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