Literature DB >> 2108669

Electrospray ionization mass spectrometric peptide mapping: a rapid, sensitive technique for protein structure analysis.

S K Chowdhury1, V Katta, B T Chait.   

Abstract

Electrospray ionization mass spectrometric peptide mapping is demonstrated to be a useful new technique for protein structure analysis. The procedure involves the digestion of the protein with trypsin and subsequent analysis of the total unfractionated digest by electrospray ionization mass spectrometry. The utility of the technique for investigating protein structure is illustrated by a peptide mapping analysis of human apolipoprotein AI (Mr = 28 kDa). The technique is rapid, sensitive, and requires no prior separation of the peptides. The discrimination effects observed in other mass spectrometric methods are less important in the present procedure.

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Year:  1990        PMID： 2108669     DOI： 10.1016/0006-291x(90)92080-j

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  12 in total

1.  Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: a new approach to protein digestion in minutes.

Authors:  Birendra N Pramanik; Urooj A Mirza; Yao Hain Ing; Yan-Hui Liu; Peter L Bartner; Patricia C Weber; Ajay K Bose
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

2.  Origin and removal of adducts (molecular mass = 98 u) attached to peptide and protein ions in electrospray ionization mass spectra.

Authors:  S K Chowdhury; V Katta; R C Beavis; B T Chait
Journal:  J Am Soc Mass Spectrom       Date:  1990-09       Impact factor: 3.109

3.  Application of nanoscale packed capillary liquid chromatography (75 μm id) and capillary zone electrophoresis/electrospray ionization mass spectrometry to the analysis of macrolide antibiotics.

Authors:  C E Parker; J R Perkins; K B Tomer; Y Shida; K O'Hara; M Kono
Journal:  J Am Soc Mass Spectrom       Date:  1992-07       Impact factor: 3.109

4.  Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry.

Authors:  D C Muddiman; X Cheng; H R Udseth; R D Smith
Journal:  J Am Soc Mass Spectrom       Date:  1996-08       Impact factor: 3.109

5.  Analysis of underivatized amino acid mixtures using high performance liquid chromatography/dual oscillating nebulizer atmospheric pressure microwave induced plasma ionization-mass spectrometry.

Authors:  J Y Kwon; M Moini
Journal:  J Am Soc Mass Spectrom       Date:  2001-01       Impact factor: 3.109

6.  Protein surface topology-probing by selective chemical modification and mass spectrometric peptide mapping.

Authors:  D Suckau; M Mak; M Przybylski
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

7.  Chemical-modification rescue assessed by mass spectrometry demonstrates that gamma-thia-lysine yields the same activity as lysine in aldolase.

Authors:  Christopher E Hopkins; Peter B O'Connor; Karen N Allen; Catherine E Costello; Dean R Tolan
Journal:  Protein Sci       Date:  2002-07       Impact factor: 6.725

8.  Structural analysis of wild-type and mutant yeast calmodulins by limited proteolysis and electrospray ionization mass spectrometry.

Authors:  S E Brockerhoff; C G Edmonds; T N Davis
Journal:  Protein Sci       Date:  1992-04       Impact factor: 6.725

9.  Spin-trapped Radicals: Determination by LC-TSP-MS and LC-ESI-MS.

Authors:  C E Parker; H Iwahashi; K B Tomer
Journal:  J Am Soc Mass Spectrom       Date:  1991-09       Impact factor: 3.109

10.  Electron detachment dissociation for top-down mass spectrometry of acidic proteins.

Authors:  Barbara Ganisl; Taras Valovka; Markus Hartl; Monika Taucher; Klaus Bister; Kathrin Breuker
Journal:  Chemistry       Date:  2011-03-23       Impact factor: 5.236
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