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Literature DB >> 24242692

Spin-trapped Radicals: Determination by LC-TSP-MS and LC-ESI-MS.

Abstract

The 4-POBN[α-(4-pyridyl-l-oxide)-N-tert-butyl-nitrone] radical adducts of ethyl and pentyl radicals were determined by a combination of high performance liquid chromatography (HPLC) combined with electron paramagnetic resonance (EPR) with HPLC-electrospray (ESI)-mass spectrometry and HPLC-thermospray (TSP)-MS. The identifIcation of the peak corresponding to the spin-trapped radical was done by performing HPLC-EPR under the same chromatographic conditions as the HPLC-MS. The radical adducts could be determined by both techniques, even though for ESI only 12 μL/min of the total 1 mL/min HPLC flow rate could be directed into the ion source.

Entities: Chemical

Year: 1991 PMID： 24242692 DOI： 10.1016/1044-0305(91)85007-S

Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN： 1044-0305 Impact factor: 3.109

14 in total

1. Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.

Authors: M A Moseley; L J Deterding; K B Tomer; J W Jorgenson
Journal: Anal Chem Date: 1991-01-15 Impact factor: 6.986

2. Electrospray interface for liquid chromatographs and mass spectrometers.

Authors: C M Whitehouse; R N Dreyer; M Yamashita; J B Fenn
Journal: Anal Chem Date: 1985-03 Impact factor: 6.986

3. Primary sequence information from intact proteins by electrospray ionization tandem mass spectrometry.

Authors: J A Loo; C G Edmonds; R D Smith
Journal: Science Date: 1990-04-13 Impact factor: 47.728

4. Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine.

Authors: A Noda; H Noda; K Ohno; T Sendo; A Misaka; Y Kanazawa; R Isobe; M Hirata
Journal: Biochem Biophys Res Commun Date: 1985-12-31 Impact factor: 3.575

5. Separation of polyunsaturated fatty acid radicals by high-performance liquid chromatography with electron spin resonance and ultraviolet detection.

Authors: R Sugata; H Iwahashi; T Ishii; R Kido
Journal: J Chromatogr Date: 1989-01-27

6. Isolation and identification of alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone radical adducts formed by the decomposition of the hydroperoxides of linoleic acid, linolenic acid, and arachidonic acid by soybean lipoxygenase.

Authors: H Iwahashi; P W Albro; S R McGown; K B Tomer; R P Mason
Journal: Arch Biochem Biophys Date: 1991-02-15 Impact factor: 4.013

4. Tandem mass spectrometry study of C-phenyl-N-tert-butyl nitrone spin adducts from in vitro rat liver microsomal metabolism of bromotrichloromethane and carbon tetrachloride.

Authors: E G Janzen; H Sang; Y Kotake; C M Dubose; J L Poyer; M Arimura
Journal: J Am Soc Mass Spectrom Date: 1995-09 Impact factor: 3.109

4 in total

Spin-trapped Radicals: Determination by LC-TSP-MS and LC-ESI-MS.

1. Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.

2. Electrospray interface for liquid chromatographs and mass spectrometers.

3. Primary sequence information from intact proteins by electrospray ionization tandem mass spectrometry.

4. Spin trapping of a free radical intermediate formed during microsomal metabolism of hydrazine.

5. Separation of polyunsaturated fatty acid radicals by high-performance liquid chromatography with electron spin resonance and ultraviolet detection.

6. Isolation and identification of alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone radical adducts formed by the decomposition of the hydroperoxides of linoleic acid, linolenic acid, and arachidonic acid by soybean lipoxygenase.

7. The catalytic mechanism of cytochrome P-450. Spin-trapping evidence for one-electron substrate oxidation.

8. Ion-spray mass spectrometry of marine neurotoxins.

9. Carbon radicals in the metabolism of alkyl hydrazines.

10. Phenyl radical production during the oxidation of phenylhydrazine and in phenylphydrazine-induced haemolysis.

1. Identification of oxidation products and free radicals of tryptophan by mass spectrometry.

2. Detection and characterization of hydroxyl radical adducts by mass spectrometry.

3. Detection and characterization by mass spectrometry of radical adducts produced by linoleic acid oxidation.

4. Tandem mass spectrometry study of C-phenyl-N-tert-butyl nitrone spin adducts from in vitro rat liver microsomal metabolism of bromotrichloromethane and carbon tetrachloride.