AIMS: To evaluate the microbiota of endodontic infections in deciduous teeth by Checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). METHODOLOGY: Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without radiographically detectable periradicular/interradicular bone resorption were collected and 32 were analysed, with three individuals contributing two samples; these were MDA-amplified and analysed by Checkerboard DNA-DNA hybridization for levels of 83 bacterial taxa. Two outcome measures were used: the percentage of teeth colonized by each species and the mean proportion of each bacterial taxon present across all samples. RESULTS: The mean amount of DNA in the samples prior to amplification was 5.2 (±4.7) ng and 6.1 (±2.3) μg after MDA. The mean number of species detected per sample was 19 (±4) (range: 3-66) to the nearest whole number. The most prevalent taxa were Prevotella intermedia (96.9%), Neisseria mucosa (65.6%), Prevotella nigrescens (56.2%) and Tannerella forsythia (56.2%). Aggregatibacter (Haemophilus) aphrophilus and Helicobacter pylori were not detected. P. intermedia (10%), Prevotella tannerae (7%) and Prevotella nigrescens (4.3%) presented the highest mean proportions of the target species averaged across the positive samples. CONCLUSION: Root canals of infected deciduous teeth had a diverse bacterial population. Prevotella sp. were commonly found with P. intermedia, Prevotella tannerae and Prevotella nigrescens amongst the most prominent species detected.
AIMS: To evaluate the microbiota of endodontic infections in deciduous teeth by Checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). METHODOLOGY: Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without radiographically detectable periradicular/interradicular bone resorption were collected and 32 were analysed, with three individuals contributing two samples; these were MDA-amplified and analysed by Checkerboard DNA-DNA hybridization for levels of 83 bacterial taxa. Two outcome measures were used: the percentage of teeth colonized by each species and the mean proportion of each bacterial taxon present across all samples. RESULTS: The mean amount of DNA in the samples prior to amplification was 5.2 (±4.7) ng and 6.1 (±2.3) μg after MDA. The mean number of species detected per sample was 19 (±4) (range: 3-66) to the nearest whole number. The most prevalent taxa were Prevotella intermedia (96.9%), Neisseria mucosa (65.6%), Prevotella nigrescens (56.2%) and Tannerella forsythia (56.2%). Aggregatibacter (Haemophilus) aphrophilus and Helicobacter pylori were not detected. P. intermedia (10%), Prevotella tannerae (7%) and Prevotella nigrescens (4.3%) presented the highest mean proportions of the target species averaged across the positive samples. CONCLUSION: Root canals of infected deciduous teeth had a diverse bacterial population. Prevotella sp. were commonly found with P. intermedia, Prevotella tannerae and Prevotella nigrescens amongst the most prominent species detected.
Authors: Frank B Dean; Seiyu Hosono; Linhua Fang; Xiaohong Wu; A Fawad Faruqi; Patricia Bray-Ward; Zhenyu Sun; Qiuling Zong; Yuefen Du; Jing Du; Mark Driscoll; Wanmin Song; Stephen F Kingsmore; Michael Egholm; Roger S Lasken Journal: Proc Natl Acad Sci U S A Date: 2002-04-16 Impact factor: 11.205
Authors: John R Nelson; Yuyang Christine Cai; Theresa L Giesler; Joseph W Farchaus; Shanmuuga T Sundaram; Maria Ortiz-Rivera; Lou P Hosta; Peter L Hewitt; J Anthony Mamone; C Palaniappan; Carl W Fuller Journal: Biotechniques Date: 2002-06 Impact factor: 1.993
Authors: M A Lana; A P Ribeiro-Sobrinho; R Stehling; G D Garcia; B K Silva; J S Hamdan; J R Nicoli; M A Carvalho; L de M Farias Journal: Oral Microbiol Immunol Date: 2001-04
Authors: B P F A Gomes; E T Pinheiro; C R Gadê-Neto; E L R Sousa; C C R Ferraz; A A Zaia; F B Teixeira; F J Souza-Filho Journal: Oral Microbiol Immunol Date: 2004-04
Authors: L C N Brito; A P Ribeiro Sobrinho; R P Teles; S S Socransky; A D Haffajee; L Q Vieira; F R F Teles Journal: Oral Dis Date: 2012-02-15 Impact factor: 3.511
Authors: Elerson Gaetti-Jardim; Luis Fernando Landucci; Kathlenn Liezbeth de Oliveira; Iracy Costa; Robson Varlei Ranieri; Ana Cláudia Okamoto; Christiane Marie Schweitzer Journal: Int J Dent Date: 2012-07-08
Authors: Natalia I Chalmers; Kevin Oh; Christopher V Hughes; Nooruddin Pradhan; Eleni Kanasi; Ygal Ehrlich; Floyd E Dewhirst; Anne C R Tanner Journal: J Oral Microbiol Date: 2015-02-03 Impact factor: 5.474
Authors: Eimear Hurley; Maurice P J Barrett; Martin Kinirons; Helen Whelton; C Anthony Ryan; Catherine Stanton; Hugh M B Harris; Paul W O'Toole Journal: BMC Oral Health Date: 2019-01-14 Impact factor: 2.757