Literature DB >> 2106882

ADP-ribosylation of the rho/rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells.

T Nishiki1, S Narumiya, N Morii, M Yamamoto, M Fujiwara, Y Kamata, G Sakaguchi, S Kozaki.   

Abstract

Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho/rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyltransferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho/rac proteins by ADP-ribosylation to induce these changes in the cells.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2106882     DOI： 10.1016/0006-291x(90)91760-p

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  31 in total

1.  Phosphatidylinositol 3-kinase, Cdc42, and Rac1 act downstream of Ras in integrin-dependent neurite outgrowth in N1E-115 neuroblastoma cells.

Authors:  S Sarner; R Kozma; S Ahmed; L Lim
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

2.  Inactivation of Rho signaling pathway promotes CNS axon regeneration.

Authors:  M Lehmann; A Fournier; I Selles-Navarro; P Dergham; A Sebok; N Leclerc; G Tigyi; L McKerracher
Journal:  J Neurosci       Date:  1999-09-01       Impact factor: 6.167

3.  Role of Cdc42 in neurite outgrowth of PC12 cells and cerebellar granule neurons.

Authors:  Ijaz Ahmed; Yolanda Calle; Shintaro Iwashita; Alam Nur-E-Kamal
Journal:  Mol Cell Biochem       Date:  2006-01       Impact factor: 3.396

4.  Purification and characterization of ADP-ribosyltransferases (exoenzyme C3) of Clostridium botulinum type C and D strains.

Authors:  K Moriishi; B Syuto; N Yokosawa; K Oguma; M Saito
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

5.  Ras-GAP controls Rho-mediated cytoskeletal reorganization through its SH3 domain.

Authors:  V Leblanc; B Tocque; I Delumeau
Journal:  Mol Cell Biol       Date:  1998-09       Impact factor: 4.272

6.  Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus.

Authors:  T Furuyashiki; K Fujisawa; A Fujita; P Madaule; S Uchino; M Mishina; H Bito; S Narumiya
Journal:  J Neurosci       Date:  1999-01-01       Impact factor: 6.167

7.  Rho family GTPases and neuronal growth cone remodelling: relationship between increased complexity induced by Cdc42Hs, Rac1, and acetylcholine and collapse induced by RhoA and lysophosphatidic acid.

Authors:  R Kozma; S Sarner; S Ahmed; L Lim
Journal:  Mol Cell Biol       Date:  1997-03       Impact factor: 4.272

8.  Activation of Rho-dependent cell spreading and focal adhesion biogenesis by the v-Crk adaptor protein.

Authors:  Z F Altun-Gultekin; S Chandriani; C Bougeret; T Ishizaki; S Narumiya; P de Graaf; P Van Bergen en Henegouwen; H Hanafusa; J A Wagner; R B Birge
Journal:  Mol Cell Biol       Date:  1998-05       Impact factor: 4.272

9.  Characterization of botulinum C3-catalyzed ADP-ribosylation of rho proteins and identification of mammalian C3-like ADP-ribosyltransferase.

Authors:  T Maehama; N Sekine; H Nishina; K Takahashi; T Katada
Journal:  Mol Cell Biochem       Date:  1994-09       Impact factor: 3.396

Review 10.  Clostridial ADP-ribosylating toxins: effects on ATP and GTP-binding proteins.

Authors:  K Aktories
Journal:  Mol Cell Biochem       Date:  1994-09       Impact factor: 3.396
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.