AIMS: The rapidly growing industrial and medical use of nanomaterials, especially zinc oxide and titanium dioxide, has led to growing concerns about their toxicity. Accordingly, the intrinsic genotoxic and cytotoxic potential of these nanoparticles have been evaluated. MATERIALS & METHODS: Using a HEp-2 cell line, cytotoxicity was tested along with mitochondrial activity and neutral red uptake assays. The genotoxic potential was determined using the Comet and the cytokinesis-blocked micronucleus assays. In addition, tyrosine phosphorylation events were investigated. RESULTS & CONCLUSION: We found concentration- and time-dependent cytotoxicity and an increase in DNA and cytogenetic damage with increasing nanoparticle concentrations. Mainly for zinc oxide, genotoxicity was clearly associated with an increase in tyrosine phosphorylation. Our results suggest that both types of nanoparticles can be genotoxic over a range of concentrations without being cytotoxic.
AIMS: The rapidly growing industrial and medical use of nanomaterials, especially zinc oxide and titanium dioxide, has led to growing concerns about their toxicity. Accordingly, the intrinsic genotoxic and cytotoxic potential of these nanoparticles have been evaluated. MATERIALS & METHODS: Using a HEp-2 cell line, cytotoxicity was tested along with mitochondrial activity and neutral red uptake assays. The genotoxic potential was determined using the Comet and the cytokinesis-blocked micronucleus assays. In addition, tyrosine phosphorylation events were investigated. RESULTS & CONCLUSION: We found concentration- and time-dependent cytotoxicity and an increase in DNA and cytogenetic damage with increasing nanoparticle concentrations. Mainly for zinc oxide, genotoxicity was clearly associated with an increase in tyrosine phosphorylation. Our results suggest that both types of nanoparticles can be genotoxic over a range of concentrations without being cytotoxic.
Authors: Maged Younes; Gabriele Aquilina; Laurence Castle; Karl-Heinz Engel; Paul Fowler; Maria Jose Frutos Fernandez; Peter Fürst; Ursula Gundert-Remy; Rainer Gürtler; Trine Husøy; Melania Manco; Wim Mennes; Peter Moldeus; Sabina Passamonti; Romina Shah; Ine Waalkens-Berendsen; Detlef Wölfle; Emanuela Corsini; Francesco Cubadda; Didima De Groot; Rex FitzGerald; Sara Gunnare; Arno Christian Gutleb; Jan Mast; Alicja Mortensen; Agnes Oomen; Aldert Piersma; Veronika Plichta; Beate Ulbrich; Henk Van Loveren; Diane Benford; Margherita Bignami; Claudia Bolognesi; Riccardo Crebelli; Maria Dusinska; Francesca Marcon; Elsa Nielsen; Josef Schlatter; Christiane Vleminckx; Stefania Barmaz; Maria Carfí; Consuelo Civitella; Alessandra Giarola; Ana Maria Rincon; Rositsa Serafimova; Camilla Smeraldi; Jose Tarazona; Alexandra Tard; Matthew Wright Journal: EFSA J Date: 2021-05-06
Authors: Young Hee Kim; Kyung A Kwak; Tae Sung Kim; Ji Hyeon Seok; Hang Sik Roh; Jong-Kwon Lee; Jayoung Jeong; Eun Ho Meang; Jeong-Sup Hong; Yun Seok Lee; Jin Seok Kang Journal: Toxicol Res Date: 2015-06
Authors: Megan J Osmond-McLeod; Ronald I W Osmond; Yalchin Oytam; Maxine J McCall; Bryce Feltis; Alan Mackay-Sim; Stephen A Wood; Anthony L Cook Journal: Part Fibre Toxicol Date: 2013-10-21 Impact factor: 9.400